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Method for DNA sequencing and gene identification

  • US 20030143549A1
  • Filed: 01/29/2002
  • Published: 07/31/2003
  • Est. Priority Date: 01/29/2002
  • Status: Abandoned Application
First Claim
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1. A method for single molecule identification of a target DNA molecule in a random coil state comprising the following steps:

  • a) attaching an optically distinguishable material to a DNA sequence recognition unit;

    b) hybridizing said DNA sequence recognition unit to said target DNA molecule in a random coil state to form a hybridized DNA complex in a random coil state;

    c) stretching said hybridized DNA complex in a random coil state to form a hybridized DNA complex in a substantially linear configuration; and

    d) detecting said optically distinguishable material in a sequential manner along said substantially linear hybridized DNA complex, thereby identifying said target DNA molecule.

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