Method for labeling and fragmenting DNA
First Claim
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1. A method for labeling and fragmenting a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:
- fragmenting the DNA by creating at least one abasic site on said DNA, attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment.
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Abstract
The invention relates to a method for labeling and fragmenting a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:
chemically fragmenting the DNA by creating at least one abasic site on said DNA,
attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment.
The invention finds a preferred application in the field of diagnosis.
31 Citations
28 Claims
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1. A method for labeling and fragmenting a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:
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fragmenting the DNA by creating at least one abasic site on said DNA, attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification