Addressable protein arrays
First Claim
1. A solid support having a linker layer on its surface, said linker layer being linked to an array of immobilized capture probes, each of said capture probes comprising a non-nucleosidic spacer group located proximal to the linker layer and an oligonucleotide sequence located distal to the linker layer, wherein said oligonucleotide sequence is bound to a nucleic acid-protein fusion.
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Accused Products
Abstract
Disclosed herein are arrays of nucleic acid-protein fusions which are immobilized to a solid surface through capture probes which include a non-nucleosidic spacer group and an oligonucleotide sequence to which the fusion (such as an RNA-protein fusion) is bound. Also disclosed herein are solid supports on which these arrays are immobilized as well as methods for their preparation and use (for example, for screening for protein-compound interactions such as protein-therapeutic compound interactions).
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Citations
29 Claims
- 1. A solid support having a linker layer on its surface, said linker layer being linked to an array of immobilized capture probes, each of said capture probes comprising a non-nucleosidic spacer group located proximal to the linker layer and an oligonucleotide sequence located distal to the linker layer, wherein said oligonucleotide sequence is bound to a nucleic acid-protein fusion.
- 23. A solid support having a linker layer on its surface, said linker layer being linked to an array of immobilized capture probes, wherein each of said capture probes is attached to the linker layer of said solid support through a non-nucleosidic spacer group, and wherein each of said capture probes comprises an oligonucleotide sequence to which a nucleic acid-protein fusion is bound.
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25. A solid support comprising an array of immobilized capture probes, each of said capture probes comprising a non-nucleosidic spacer group and an oligonucleotide sequence to which a ribosome display particle is bound.
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26. A method for preparing a solid support, said method comprising the steps of:
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(a) preparing a capture probe by linking a spacer group to an oligonucleotide sequence;
(b) attaching said capture probe to said solid support; and
(c) binding a nucleic acid-protein fusion to said capture probe. - View Dependent Claims (27)
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28. A method for preparing a solid support, said method comprising the steps of:
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(a) attaching a spacer group to a surface of said solid support;
(b) attaching a bifunctional linker to said spacer group;
(c) attaching a capture probe to said bifunctional linker; and
(d) binding a nucleic acid-protein fusion to said capture probe. - View Dependent Claims (29)
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Specification