Proteins having DNA repair promoting activity

• US 20030143707A1
• Filed: 08/13/2002
• Published: 07/31/2003
• Est. Priority Date: 08/14/2001
• Status: Abandoned Application

• Current Assignee: Japan Atomic Energy Research Institute
• Original Assignee: Japan Atomic Energy Research Institute
• Inventors: Watanabe, Hiroshi, Cui, Suzhen, Kitayama, Shigeru, Narumi, Issay, Satoh, Katsuya
• Application Number:
  US10/216,862
• Publication Number:
  US 20030143707A1
• Time in Patent Office: Days
• US Class Current: 435/183
• CPC Class Codes: C07K 14/195 from bacteria

Abstract

Provided are a protein having DNA repair reaction promoting activity which consists of the amino acid sequence represented by SEQ ID NO: 1 or a modified product of that amino acid sequence, a nucleotide sequence coding for said protein having DNA repair promoting activity, a recombinant DNA having said nucleotide sequence inserted into vector DNA, a transformant containing said recombinant DNA, a process for producing a protein having DNA repair promoting activity comprising the steps of cultivating said transformant in a medium and harvesting a protein having DNA repair promoting activity from the culture, and an antibody binding to said protein.

Claims

1. A protein having DNA repair promoting activity which consists of the amino acid sequence represented by SEQ ID NO:

1 or an amino acid sequence that is identical to the amino acid sequence represented by SEQ ID NO;

1 except that it undergoes deletion, substitution or addition of one or more amino acids.

7. A recombinant vector containing DNA having a nucleotide sequence coding for the protein according to any one of claims 1-3.

9. A transformant containing the recombinant vector containing DNA having a nucleotide sequence coding for the protein according to any one of claims 1-3 or the DNA according to any one of claims 4-6.

10. A process for producing a protein having DNA repair promoting activity comprising the steps of cultivating the transformant according to claim 9 in a medium and harvesting a protein having DNA repair promoting activity from the culture.

11. An antibody that binds to the protein according to any one of claims 1-3.

2. A protein having DNA repair promoting activity that consists of the amino acid sequence represented by SEQ ID NO:

1 or an amino acid sequence encoded by a nucleotide sequence hybridizable under stringent conditions with a nucleotide sequence complementary to a nucleotide sequence that codes for the amino acid sequence represented by SEQ ID NO;

1.

3. A protein having DNA repair promoting activity which consists of the amino acid sequence represented by SEQ ID NO:

1 or an amino acid sequence having at least 60% amino acid sequence homology to the amino acid sequence represented by SEQ ID NO;

1.

4. DNA that has DNA consisting of the nucleotide sequence represented by SEQ ID NO:

2 or a nucleotide sequence identical to the nucleotide sequence represented by SEQ ID NO;

2 except that it undergoes deletion, substitution or addition of one or more bases and which codes for a protein having DNA repair promoting activity.

8. A recombinant vector containing the DNA according to any one of claims 4-6.

5. DNA that has DNA consisting of the nucleotide sequence represented by SEQ ID NO:

2 or a nucleotide sequence hybridizable under stringent conditions with a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO;

2 and which codes for a protein having DNA repair promoting activity.

6. DNA that has DNA consisting of a nucleotide sequence having at least 60% amino acid sequence homology to the nucleotide sequence represented by SEQ ID NO:

2 and which codes for a protein having DNA repair promoting activity.