Peptide-enhanced transfections
First Claim
1. A composition for transfecting a cell which comprises one or more nucleic acid molecules, one or more peptides or proteins, and one or more transfection agents.
6 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
-
Citations
77 Claims
- 1. A composition for transfecting a cell which comprises one or more nucleic acid molecules, one or more peptides or proteins, and one or more transfection agents.
- 42. A composition for transfecting a cell which comprises a component of transfection agent covalently linked to a peptide or protein.
- 48. A composition for transfecting a cell obtained by combining one or more nucleic acid molecules, one or more peptides or proteins, and one or more transfection agents.
-
55. A method for transfecting a cell with a nucleic acid, the method comprising the steps:
-
(a) admixing one or more peptides or proteins with a nucleic acid to form a peptide-nucleic acid complex or a protein-nucleic acid complex;
(b) adding a transfection agent to the complex from step (a) to obtain an aggregate of the transfection agent and said complex; and
(c) contacting said cell with the aggregate from step (b). - View Dependent Claims (56, 57, 58, 59, 60, 61, 62, 63)
-
- 64. A transfection reagent kit which comprises a transfection agent and a peptide or protein or a modified peptide or protein capable of enhancing transfection of the transfection agent.
- 72. A peptide comprising an NLS sequence modified by covalent bonding to a nucleic acid-binding group.
- 75. A peptide comprising a Tat sequence modified by covalent bonding to a nucleic acid-binding group.
Specification