Starch biosynthetic enzymes

• US 20030145353A1
• Filed: 01/02/2003
• Published: 07/31/2003
• Est. Priority Date: 05/07/1997
• Status: Abandoned Application

• Current Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Allen, Stephen M., Thorpe, Catherine J., Lightner, Jonathan E.
• Application Number:
  US10/336,587
• Publication Number:
  US 20030145353A1
• Time in Patent Office: Days
• US Class Current: 800/284
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
• CPC Class Codes: C12N 15/8273 for drought, cold, salt resistance
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)

Abstract

This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a plant glycogenin or water stress protein. The invention also relates to the construction of chimeric genes encoding all or a portion of a plant glycogenin or water stress protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of a plant glycogenin or water stress protein in a transformed host cell.

Claims

1. An isolated polynucleotide comprising:

(a) a nucleotide sequence encoding a glycogenin, wherein the amino acid sequence of the glycogenin and the amino acid sequence of SEQ ID NO;

18, SEQ ID NO;

20, SEQ ID NO;

22, or SEQ ID NO;

24 have at least 90% identity based on the Clustal alignment method, or

(b) the complement of the nucleotide sequence.

2. The polynucleotide of claim 1, wherein the amino acid sequence of the glycogenin and the amino acid sequence of SEQ ID NO:

18, SEQ ID NO;

20, SEQ ID NO;

22, or SEQ ID NO;

24 have at least 95% identity based on the Clustal alignment method.

3. The polynucleotide of claim 1, wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:

17, SEQ ID NO;

19, SEQ ID NO;

21, or SEQ ID NO;

23.

4. The polynucleotide of claim 1, wherein the glycogenin comprises the amino acid sequence of SEQ ID NO:

18, SEQ ID NO;

20, SEQ ID NO;

22, or SEQ ID NO;

24.

5. A chimeric gene comprising the polynucleotide of claim 1 operably linked to a regulatory sequence.

6. A method for transforming a cell comprising transforming a cell with the polynucleotide of claim 1.

7. A cell comprising the chimeric gene of claim 5.

8. A method for producing a plant comprising transforming a plant cell with the polynucleotide of claim 1 and regenerating a plant from the transformed plant cell.

9. A plant comprising the chimeric gene of claim 5.

10. A seed comprising the chimeric gene of claim 5.