Systems for analyzing microtissue arrays
First Claim
1. A method comprising:
- capturing an image of a tissue microarray at a first magnification, the tissue microarray including a plurality of disks, each disk including a sample of a biological specimen;
locating a first group of disks from the plurality of disks using one or more visual features of the first group of disks;
identifying a grid defined by the first group of disks;
locating a second group of disks from the plurality of disks using the identified grid to locate disks which do not include the one or more visual features of the first group of disks;
capturing an image of each one of the first group of disks and the second group of disks at a second magnification; and
storing the captured images.
2 Assignments
0 Petitions
Accused Products
Abstract
The systems described herein autonomously image, analyze, and store date for samples in a tissue microarray. The system may include a tissue microarray, a robotic microscope, and an imaging workstation that executes software to automatically control operation of the microscope to capture images from the microarray and analyze image results. A low magnification may be used to register samples within the microarray and obtain coordinates for each tissue specimen. Progressively higher magnifications may be used to analyze images of each registered specimen. Images and quantitative data from the images may then be stored in a relational database for subsequent review. The system may be local, or may be Web-based for distributed control and sharing of results.
34 Citations
27 Claims
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1. A method comprising:
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capturing an image of a tissue microarray at a first magnification, the tissue microarray including a plurality of disks, each disk including a sample of a biological specimen;
locating a first group of disks from the plurality of disks using one or more visual features of the first group of disks;
identifying a grid defined by the first group of disks;
locating a second group of disks from the plurality of disks using the identified grid to locate disks which do not include the one or more visual features of the first group of disks;
capturing an image of each one of the first group of disks and the second group of disks at a second magnification; and
storing the captured images. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A computer program product comprising:
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computer executable code for controlling an imaging device to capture an image of a tissue microarray at a first magnification, the tissue microarray including a plurality of disks, each disk including a sample of a biological specimen;
computer executable code for locating a first group of disks from the plurality of disks using one or more visual features of the first group of disks;
computer executable code for identifying a grid defined by the first group of disks;
computer executable code for locating a second group of disks from the plurality of disks using the identified grid to locate disks which do not include the one or more visual features of the first group of disks;
computer executable code for controlling the imaging device to capture an image of each one of the first group of disks and the second group of disks at a second magnification; and
computer executable code for storing the captured images. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. A system comprising:
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capturing means for capturing an image of a tissue microarray at a first magnification, the tissue microarray including a plurality of disks, each disk including a sample of a biological specimen;
first locating means for locating a first group of disks from the plurality of disks using one or more visual features of the first group of disks;
identifying means for identifying a grid defined by the first group of disks;
second locating means for locating a second group of disks from the plurality of disks using the identified grid to locate disks which do not include the one or more visual features of the first group of disks;
the capturing means further for capturing an image of each one of the first group of disks and the second group of disks at a second magnification; and
storing means for storing the captured images.
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- 22. A system comprising an imaging device and a computer, the imaging device responsive to control signals to provide images at a specified magnification and a specified location within a tissue microarray, and the computer providing the control signals to the imaging device and receiving the images provided by the imaging device, the computer configured to locate a first group of disks on the tissue microarray within an image of the tissue microarray captured at a first magnification by applying a convolution of the image of the tissue microarray with a template, and the computer further configured to locate a second group of disks on the tissue microarray within the image of the tissue microarray using the coordinates of a grid obtained from the first group of disks.
Specification