Red blood cell resuspension solution and method
First Claim
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1. An aqueous red blood cell storage solution comprising sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol, and having a pH of approximately 7.4.
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Abstract
The present invention provides an improved aqueous solution for suspending and storing red blood cells. The solution includes sodium citrate, a combination of sodium biphosphate and sodium phosphate dibasic, adenine, and mannitol. The solution is formulated at a low osmolarity and has a physiological pH of approximately 7.4. The solution simultaneously maintains stored red cells ATP and 2,3-BPG.
41 Citations
36 Claims
- 1. An aqueous red blood cell storage solution comprising sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol, and having a pH of approximately 7.4.
- 5. An aqueous red blood cell storage solution comprising sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol, and having an osmolarity of less than 300 mOsm/l.
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9. An aqueous red blood cell storage solution comprising a first distinct solution and a second distinct solution wherein:
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the first solution includes in millimolar concentration (mmol/l) approximately 20 mmol/l to about 140 mmol/l of at least one sugar chosen from the group consisting of dextrose and fructose;
the second solution includes in millimolar concentrations;
approximately 1 mmol/l to about 2.2 mmol/l adenine;
approximately 20 mmol/l to about 110 mmol/l mannitol;
approximately 2.2 mmol/l to about 90 mmol/l sodium citrate;
approximately 1 mmol/l to about 10 mmol/l sodium biphosphate;
approximately 5 mmol/l to about 25 mmol/l sodium phosphate dibasic; and
approximately 0 mmol/l to about 2 mmol/ guanosine. - View Dependent Claims (10, 11, 12)
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13. A two part aqueous red blood cell storage solution comprising:
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a first part including sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol; and
a second part including a sugar chosen from the group consisting of dextrose and fructose. - View Dependent Claims (14, 15, 16, 17)
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18. An aqueous red blood cell storage solution comprising in millimolar concentration (mmol/l):
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approximately 30 mmol/l to about 60 mmol/l of at least one sugar chosen from the group consisting of dextrose and fructose;
approximately 1.2 mmol/l to about 1.7 mmol/l adenine;
approximately 30 mmol/l to about 50 mmol/l mannitol;
approximately 4.5 mmol/l to about 55 mmol/l sodium citrate;
approximately 2 mmol/l to about 5 mmol/l sodium biphosphate;
approximately 8 mmol/l to about 18 mmol/l sodium phosphate dibasic; and
approximately 0 mmol/l to about 1.5 mmol/l guanosine. - View Dependent Claims (19, 20)
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21. An aqueous red blood cell storage solution comprising in millimolar concentration (mmol/l):
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approximately 1.0 mmol/l to about 2.2 mmol/l adenine;
approximately 20 mmol/l to about 110 mmol/l mannitol;
approximately 2.2 mmol/l to about 90 mmol/l sodium citrate;
approximately 1 mmol/l to about 10 mmol/l sodium biphosphate;
approximately 5 mmol/l to about 25 mmol/l sodium phosphate dibasic; and
approximately 0 mmol/l to about 2.0 mmol/l guanosine; and
having a pH of approximately 7.4. - View Dependent Claims (22)
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- 23. A method of storing red blood cells comprising the step of combining a plasma depleted collection of red blood cells with an aqueous storage solution comprising sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol, and having an osmolarity of less than 300 mOsm/l.
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27. A method for storing red blood cells comprising the steps of:
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adding an anticoagulant to collected whole blood having a sufficient level of dextrose to provide a nutrient supply to separated red blood cells;
separating the red bloods cells from the whole blood; and
adding to the separated red blood cells an aqueous storage solution comprising sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol. - View Dependent Claims (28, 29, 30, 31, 32)
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- 33. A method of storing red blood cells comprising the step of combining a plasma depleted collection of red blood cells with an aqueous storage solution comprising sodium citrate, sodium biphosphate, sodium phosphate dibasic, adenine, and mannitol, and having a pH of approximately 7.4.
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36. A two part aqueous red blood cell storage solution comprising:
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a first part including at least, sodium biphosphate and sodium phosphate dibasic;
a second part including at least a sugar chosen from the group consisting of dextrose and fructose; and
at least one of the first and second parts including mannitol, sodium citrate, and adenine.
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Specification