Diagnostic microarray and method of use thereof
First Claim
1. A microarray device for the analysis of biological samples comprising:
- a liquid permeable layer including a plurality of microregions, each microregion including a plurality of microbeads embedded in the liquid permeable layer;
wherein the microbeads in a given microregion have a plurality of target probes on their surfaces.
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Accused Products
Abstract
A microarray device for the analysis of biological samples is provided. The device includes a liquid permeable layer having a plurality of microregions, each including a plurality of probe-labeled microbeads embedded in the liquid permeable layer. The microbeads in a given microregion include a plurality of the same target probes on their surfaces. The target probes are capable of specifically binding to one or more particular target molecules (e.g., nucleic acid, polypeptide, small molecule antigen). The device typically has the capability of inducing a sample solution to move through the liquid permeable layer under the influence of an applied voltage. Kits which include the device and methods of simultaneously detecting a plurality of different target molecules in a sample solution are also provided.
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Citations
40 Claims
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1. A microarray device for the analysis of biological samples comprising:
a liquid permeable layer including a plurality of microregions, each microregion including a plurality of microbeads embedded in the liquid permeable layer;
wherein the microbeads in a given microregion have a plurality of target probes on their surfaces.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of detecting one or more target molecules in a sample solution, the method comprising:
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(a) electrophoretically transporting the sample solution through a liquid permeable layer, wherein the liquid permeable layer includes at least one microregion having a plurality of microbeads embedded in the liquid permeable layer;
the microbeads having a plurality of target probes on their surfaces;
wherein the target probes are capable of specifically binding to designated target molecules to form target probe/target molecule complexes; and
(b) detecting the target probe/target molecule complexes. - View Dependent Claims (23, 24, 25, 26)
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27. A method of detecting a target molecule in a sample comprising:
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(a) introducing a first low conductivity buffer solution including the sample into a liquid chamber;
(b) electrophoretically transporting the first low conductivity buffer solution through a liquid permeable layer which is in fluid connection with the liquid chamber;
wherein the liquid permeable layer includes at least one microregion having a plurality of microbeads embedded in the liquid permeable layer;
the microbeads having a plurality of a target probe on their surfaces;
the target probe being capable of specifically binding to the target molecule to form a target molecule/target probe complex;
(c) introducing a second low conductivity buffer solution into the liquid chamber;
wherein the second low conductivity buffer solution includes a fluorescently labeled target molecule;
(d) electrophoretically transporting the second low conductivity buffer solution through the liquid permeable layer to form a fluorescent target molecule/target probe complex; and
(e) detecting the fluorescent target molecule/target probe complex. - View Dependent Claims (28)
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29. A kit for the analysis of biological samples comprising:
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(a) a microarray device comprising a liquid permeable layer including a plurality of microregions, each microregion including a plurality of microbeads embedded in the liquid permeable layer;
wherein the microbeads have a plurality of target probes on their surfaces and the microbeads in a given microregion have a plurality of the target probes on their surfaces;
(b) a low conductivity buffer solution; and
(c) a buffer solution including a set of visualization probes. - View Dependent Claims (30, 31, 32, 33, 34, 35)
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36. A microarray device for the analysis of biological samples comprising:
a liquid permeable layer including at least one microregion which includes a plurality of microbeads embedded in the liquid permeable layer;
wherein the microbeads have a plurality of target probes on their surfaces.- View Dependent Claims (37)
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38. A method of detecting a target molecule in a sample comprising:
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(a) introducing a plurality of a visualization probe into a low conductivity solution including the sample to form a labeled solution;
wherein the visualization probe is capable of specifically binding to the target molecule to form a labeled target molecule;
(b) electrophoretically transporting the labeled solution through a liquid permeable layer;
wherein the liquid permeable layer includes at least one microregion having a plurality of microbeads embedded in the liquid permeable layer;
the microbeads having a plurality of a target probe on their surfaces;
wherein the target probe is capable of specifically binding to the labeled target molecule to form a labeled target molecule/target probe complex on a microbead surface; and
(c) detecting the bound labeled target molecule/target probe complex. - View Dependent Claims (39, 40)
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Specification