Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
First Claim
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1. A method of preparing a multicolor quantum dot-tagged bead, which method comprises:
- (a) providing at least one porous polymer bead, wherein the pores of the bead are large enough to incorporate quantum dots;
(b) combining predetermined amounts of multicolor quantum dots with at least one bead; and
(c) isolating the multicolor quantum dot-tagged bead.
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Abstract
The present invention provides a method of preparing a multicolor quantum dot-tagged bead, a multicolor quantum dot-tagged bead, a conjugate thereof, and a composition comprising such a bead or conjugate. Additionally, the present invention provides a method of making a conjugate thereof and methods of using a conjugate for multiplexed analysis of target molecules.
221 Citations
65 Claims
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1. A method of preparing a multicolor quantum dot-tagged bead, which method comprises:
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(a) providing at least one porous polymer bead, wherein the pores of the bead are large enough to incorporate quantum dots;
(b) combining predetermined amounts of multicolor quantum dots with at least one bead; and
(c) isolating the multicolor quantum dot-tagged bead. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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- 17. A multicolor quantum dot-tagged bead, which comprises at least one quantum dot and a porous polymer bead, wherein the bead has pores large enough to incorporate the quantum dot, and wherein the quantum dots are present in a precisely controlled ratio.
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60. A conjugate comprising at least one quantum dot and a porous polymer bead, and a probe, wherein the bead has pores large enough to incorporate the quantum dot, wherein the quantum dots are present in a precisely controlled ratio, wherein the conjugate hybridizes to a target sequence, wherein the probe is attached to the bead, and wherein the target is labeled with a quantum dot.
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61. A method of preparing a multicolor quantum dot-tagged bead, which method comprises:
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(a) providing at least one porous bead by solvent-system polymerization that includes about 0.3-5% by volume of a cross-linking agent, wherein the pores of the bead have an average diameter of at least about 1 nm;
(b) combining predetermined amounts of multicolor quantum dots with at least one bead; and
(c) isolating the multicolor quantum dot-tagged bead. - View Dependent Claims (62, 63, 64, 65)
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Specification