Electrospraying solutions of substances for mass fabrication of chips and libraries

• US 20030150739A1
• Filed: 03/03/2003
• Published: 08/14/2003
• Est. Priority Date: 06/20/1997
• Status: Abandoned Application

• Current Assignee: New York University
• Original Assignee: New York University
• Inventors: Morozov, Victor, Morozova, Tamara Ya.
• Application Number:
  US10/376,668
• Publication Number:
  US 20030150739A1
• Time in Patent Office: Days
• US Class Current: 205/133
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00371 comprising electrodes
• CPC Class Codes: B01J 2219/0043 for direct application of reagents, e.g. through openings in a shutter
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00711 : Light-directed synthesis
• CPC Class Codes: B01J 2219/00713 : Electrochemical synthesis
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B05B 12/36 : Side shields, i.e. shields extending in a direction substantially parallel to the spray jet
• CPC Class Codes: B05B 5/002 : comprising means for neutralising the spray of charged droplets or particules
• CPC Class Codes: B05B 5/0255 : spraying and depositing by electrostatic forces only
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 33/543 : with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/545 : Synthetic resin

Abstract

A method of fabricating deposits of non-volatile substances, including biomacromolecules, in the form of spots and filing on a substrate surface by electrospray, where the deposits are used to determine the interaction of the deposited non-volatile substances to other substances. Also included in this method is the mass fabrication on a single chip of an array of single and multicomponent microsamples.

Claims

1. A dry deposit of a non-volatile biologically functional and/or biologically active substance formed on a substrate, wherein said dry deposit retains biological function and/or activity, has a thickness ranging down to a monolayer, has a homogeneity of thickness not larger than ±

10% and contains molecule clusters with a size between 5 nm and 50 nm.

2. The dry deposit of a non-volatile biologically functional and/or biologically active substance in accordance with claim 1, wherein the molecule clusters are densely packed but not completely coalesced, and said dry deposit of a non-volatile biologically functional and/or biologically active substance has intercluster channels sufficiently penetrable by ligands.

3. The dry deposit in accordance with claim 1 which is formed on a substrate by electrospraying a solution of a non-volatile biologically functional and/or biologically active substance.

4. The dry deposit of a non-volatile biologically functional and/or biologically active substance in accordance with claim 1 which is formed as a film.

5. A sample comprising a substrate, and a plurality of dry deposits of different non-volatile biologically functional and/or biologically active substances electrosprayed on said substrate, wherein each of said dry deposits is in accordance with the dry deposit of claim 1.

6. The sample in accordance with claim 5, wherein said electrosprayed dry deposits of different substances are formed at a density of two to more than 200,000 deposits per square inch.