Method for nucleotide sequencing
First Claim
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1. A method for nucleotide sequencing, comprising:
- continuously generating a reaction product in a nucleotide extension reaction of a polynucleotide chain;
detecting the reaction product; and
determining the alignment in the sequentially extending polynucleotide.
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Abstract
The present invention relates to a method for nucleotide sequencing in which a nucleotide sequence of a single nucleotide or plural nucleotides can be determined by assaying reactions in one reaction solution. The nucleotide sequence can be determined according to the present method by detecting a reaction product continuously generated in an extension reaction system following the extension reaction of polynucleotide chain and thereby determining the alignment in the sequentially extending polynucleotide.
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11 Claims
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1. A method for nucleotide sequencing, comprising:
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continuously generating a reaction product in a nucleotide extension reaction of a polynucleotide chain;
detecting the reaction product; and
determining the alignment in the sequentially extending polynucleotide. - View Dependent Claims (2, 3, 4, 5, 7, 8, 9, 10, 11)
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6. The method according to clam 4, wherein the fusion substance is a deoxyribonucleotide 5′
- triphosphate ester or a ribonucleotide 5′
triphosphate ester.
- triphosphate ester or a ribonucleotide 5′
Specification