Polyelectrolyte multilayers that influence cell growth, methods of applying them, and articles coated with them

• US 20030157260A1
• Filed: 10/23/2002
• Published: 08/21/2003
• Est. Priority Date: 10/25/2001
• Status: Abandoned Application

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Rubner, Michael F., Mendelsohn, Jonas D., Yang, Sung Y.
• Application Number:
  US10/278,774
• Publication Number:
  US 20030157260A1
• Time in Patent Office: Days
• US Class Current: 427/402
• CPC Class Codes: A61L 2420/02 Methods for coating medical devices
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 29/085 Macromolecular materials
• CPC Class Codes: A61L 31/10 Macromolecular materials

Abstract

One aspect of the present invention relates to a method of coating a surface, comprising sequentially depositing on a surface, under pH-controlled conditions, alternating layers of polymers to provide a coated surface, wherein a first polymer is selected from the group consisting of pH dependent cationic polyelectrolytes and neutral polymers, and a second polymer is selected from the group consisting of anionic polyelectrolytes, thereby permitting or preventing cell adhesion to said coated surface. In certain embodiments, the aforementioned method provides a coated surface to which cell adhesion is permitted. In certain embodiments, the aforementioned method provides a coated surface to which cell adhesion is prevented. Another aspect of the present invention relates to a method of rendering a surface cytophilic, comprising the step of coating a surface with a polyelectrolyte multilayer film, which film swells to less than or equal to about 150% of its original thickness when exposed to an aqueous medium. Another aspect of the present invention relates to a method of rendering a surface cytophobic, comprising the step of coating a surface with a polyelectrolyte multilayer film, which film swells to greater than or equal to about 200% of its original thickness when exposed to an aqueous medium.

Claims

1. A method of coating a surface, comprising sequentially depositing on a surface, under pH-controlled conditions, alternating layers of polymers to provide a coated surface, wherein a first polymer is selected from the group consisting of pH dependent cationic polyelectrolytes and neutral polymers, and a second polymer is selected from the group consisting of anionic polyelectrolytes, thereby preventing or permitting cell adhesion to said coated surface.

2. The method of claim 1, wherein cell adhesion to said coated surface is prevented.

3. The method of claim 1, wherein cell adhesion to said coated surface is permitted.

4. The method of claim 2, wherein said second polymer is a pH dependent anionic polyelectrolyte.

5. The method of claim 4, wherein said second polymer is polyacrylic acid (PAA).

6. The method of claim 4, wherein said second polymer is polymethacrylic acid (PMA).

7. The method of claim 2, wherein said first polymer is polyallylamine hydrochloride (PAH).

8. The method of claim 2, wherein said first polymer is polyacrylamide (PAAm).

9. The method of claim 3, wherein said second polymer is poly(styrene sulfonate) (SPS).

10. The method of claim 2 or 3, wherein said second polymer is a pH dependent anionic polyelectrolyte.

11. The method of claim 10, wherein said second polymer is PAA.

12. The method of claim 10, wherein said second polymer is PMA.

13. The method of claim 2 or 3, wherein said first polymer is PAH.

14. The method of claim 4, wherein said first polymer is PAH;

and said second polymer is PAA.

15. The method of claim 4, wherein said first polymer is PAH;

and said second polymer is PMA.

16. The method of claim 4, wherein said first polymer is PAAm;

and said second polymer is PAA.

17. The method of claim 4, wherein said first polymer is PAAm;

and said second polymer is PMA.

18. The method of claim 3, wherein said first polymer is PAH;

and said second polymer is SPS.

19. The method of claim 10, wherein said first polymer is PAH;

and said second polymer is PAA.

20. The method of claim 10, wherein said first polymer is PAH;

and said second polymer is PMA.

21. The method of claim 2, wherein said first polymer is a pH dependent cationic polyelectrolyte deposited at a pH between about 2.0 and about 2.5;

and said second polymer is deposited at a pH between about 2.0 and about 2.5.

22. The method of any of claims 4, 5, or 6, wherein said first polymer is a pH dependent cationic polyelectrolyte deposited at a pH between about 2.0 and about 2.5;

and said second polymer is deposited at a pH between about 2.0 and about 2.5.

23. The method of claim 14 or 15, wherein the PAH is deposited at a pH between about 2.0 and about 2.5;

and said PAA or PMA is deposited at a pH between about 2.0 and about 2.5.

24. The method of claim 14 or 15, wherein the PAH is deposited at a pH of about 2.5;

and

said PAA or PMA is deposited at a pH of about 2.5.

25. The method of claim 3, wherein said first polymer is a pH dependent cationic polyelectrolyte deposited at a pH of about 7.5;

and said second polymer is PAA deposited at a pH of about 3.5.

26. The method of claim 3, wherein said first polymer is a pH dependent cationic polyelectrolyte deposited at a pH of about 6.5;

and said second polymer is PAA deposited at a pH of about 6.5.

27. The method of claim 3, wherein said first polymer is a pH dependent cationic polyelectrolyte deposited at a pH of about 4.5;

and said second polymer is PMA deposited at a pH of about 4.5.

28. The method of claim 3, wherein said first polymer is a pH dependent cationic polyelectrolyte deposited at a pH of about 6.5;

and said second polymer is PMA deposited at a pH of about 6.5.

29. The method of claim 3, wherein said first polymer is PAH deposited at a pH of about 7.5;

and said second polymer is PAA deposited at a pH of about 3.5.

30. The method of claim 3, wherein said first polymer is PAH deposited at a pH of about 6.5;

and said second polymer is PAA deposited at a pH of about 6.5.

31. The method of claim 3, wherein said first polymer is PAH deposited at a pH of about 4.5;

and said second polymer is PMA deposited at a pH of about 4.5.

32. The method of claim 3, wherein said first polymer is PAH deposited at a pH of about 6.5;

and said second polymer is PMA deposited at a pH of about 6.5.

33. The method of claim 16, wherein the PAAm is deposited at a pH between about 2.5 and about 3.5;

and the PAA is deposited at a pH between about 2.5 and about 3.5.

34. The method of claim 17, wherein the PAAm is deposited at a pH between about 2.5 and about 3.5;

and the PMA is deposited at a pH between about 2.5 and about 3.5.

35. The method of claim 16, wherein the PAAm is deposited at a pH of about 3.0;

and the PAA is deposited at a pH of about 3.0.

36. The method of claim 17, wherein the PAAm is deposited at a pH of about 3.0;

and the PMA is deposited at a pH of about 3.0.

37. The method of claim 16, 17, 33, 34, 35, or 36, further comprising heating the coated surface at about 95°

C. for about 8-12 hours.

38. An article coated by the method of claim 1.

39. An article coated by the method of claim 2.

40. An article coated by the method of claim 3.

41. An article coated by the method of claim 14.

42. An article coated by the method of claim 15.

43. An article coated by the method of claim 16.

44. An article coated by the method of claim 17.

45. An article coated by the method of claim 18.

46. An article coated by the method of claim 19.

47. An article coated by the method of claim 20.

48. An article coated by the method of claim 37.

49. The article of claim 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48, wherein said article is selected from the group consisting of blood vessel stents, angioplasty balloons, vascular graft tubing, prosthetic blood vessels, vascular shunts, heart valves, artificial heart components, pacemakers, pacemaker electrodes, pacemaker leads, ventricular assist devices, contact lenses, intraocular lenses, sponges for tissue engineering, foams for tissue engineering, matrices for tissue engineering, scaffolds for tissue engineering, biomedical membranes, dialysis membranes, cell-encapsulating membranes, drug delivery reservoirs, drug delivery matrices, drug delivery pumps, catheters, tubing, cosmetic surgery prostheses, orthopedic prostheses, dental prostheses, wound dressings, sutures, soft tissue repair meshes, percutaneous devices, diagnostic biosensors, cellular arrays, cellular networks, microfluidic devices, and protein arrays.

50. A method of rendering a surface cytophilic, comprising the step of coating a surface with a polyelectrolyte multilayer film, which film swells to less than or equal to about 150% of its original thickness when exposed to an aqueous medium.

52. An article coated according to the method of claim 50.

54. The article of claim 52 or 53, wherein said article is selected from the group consisting of blood vessel stents, angioplasty balloons, vascular graft tubing, prosthetic blood vessels, vascular shunts, heart valves, artificial heart components, pacemakers, pacemaker electrodes, pacemaker leads, ventricular assist devices, contact lenses, intraocular lenses, sponges for tissue engineering, foams for tissue engineering, matrices for tissue engineering, scaffolds for tissue engineering, biomedical membranes, dialysis membranes, cell-encapsulating membranes, drug delivery reservoirs, drug delivery matrices, drug delivery pumps, catheters, tubing, cosmetic surgery prostheses, orthopedic prostheses, dental prostheses, wound dressings, sutures, soft tissue repair meshes, percutaneous devices, diagnostic biosensors, cellular arrays, cellular networks, microfluidic devices, and protein arrays.

51. A method of rendering a surface cytophobic, comprising the step of coating a surface with a polyelectrolyte multilayer film, which film swells to greater than or equal to about 200% of its original thickness when exposed to an aqueous medium.

53. An article coated according to the method of claim 51.