Cryopreservation of selected sperm cells
First Claim
1. A method for the cryopreservation of sperm comprising:
- (a) obtaining a selected sperm sample;
(b) cooling said selected sperm sample;
(c) isolating sperm from said selected sperm sample to produce isolated sperm;
(d) adding final extender to said isolated sperm to produce a suspension of sperm; and
(e) freezing said suspension of sperm.
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Accused Products
Abstract
The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.
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Citations
37 Claims
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1. A method for the cryopreservation of sperm comprising:
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(a) obtaining a selected sperm sample;
(b) cooling said selected sperm sample;
(c) isolating sperm from said selected sperm sample to produce isolated sperm;
(d) adding final extender to said isolated sperm to produce a suspension of sperm; and
(e) freezing said suspension of sperm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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- 27. A frozen selected sperm sample comprising a portion of the sperm present in a source sample, said portion of sperm selected for a characteristic.
Specification