Component comprising a plurality of fiber elements and sample molecules that are immobilized on said fiber elements

• US 20030162232A1
• Filed: 03/03/2003
• Published: 08/28/2003
• Est. Priority Date: 02/28/2000
• Status: Abandoned Application

• Current Assignee: Variom Biotechnology AG
• Original Assignee: Variom Biotechnology AG
• Inventors: Ozkan, Derya
• Application Number:
  US10/220,334
• Publication Number:
  US 20030162232A1
• Time in Patent Office: Days
• US Class Current: 435/7.9
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G02B 6/02033 Core or cladding made from organic material, e.g. polymeric material G02B1/04 takes precedence
• CPC Class Codes: G02B 6/06 the relative position of the fibres being the same at both ends, e.g. for transporting images

Abstract

The invention relates to a component comprising a plurality of fiber elements and sample molecules of a selected sample molecule species or of selected sample molecule species groups, said sample molecules being immobilized on said fiber elements, whereby a specific sample molecule species or sample molecule species group is assigned to each fiber element. The invention is characterized in that the sample molecules are immobilized on outer surfaces of the fiber elements, and in that a supporting element fixes the fiber elements in an interspaced manner and in a radial direction with regard to the fiber elements or the fiber elements are bundled together with linear contact.

Claims

1. A component comprising a plurality of fiber elements and sample molecules of selected sample molecule species or selected sample molecule species groups that are immobilized on said fiber elements, to each fiber element being associated a specific sample molecule species or sample molecule species group,

characterized by

that the sample molecules are immobilized on outer surfaces of the fiber elements, and

that the fiber elements are fixed by means of a supporting element in a radial direction with regard to the fiber elements in an interspaced manner or are bundled together with linear contact.

2. A component according to claim 1, characterized by that the fiber elements are arranged parallely to each other as fiber element bundles.

3. A component according to claim 1 or 2, characterized by that the fiber elements are optical fiber elements.

4. A component according to one of claims 1 to 3, characterized by that the fiber elements are made from a polymeric material, preferably selected from the group consisting of “

polyethylene (PE), polycarbonate (PC), polyvinyl chloride (PVC), polystyrene (PS), polyethylene terephthalate (PETP), polyethersulfone (PES), polyetherether ketone (PEEK), polyphenylene oxide (PPO), polyphenylene sulfide (PPS), polybutylene terephthalate (PBT), polyoxymethylene (POM), polysulfone (PSU), polyetherimide (PEI), polyamide (PA) and mixtures and copolymers of the monomers of such polymers”

, in particular selected from the group consisting of “

polycarbonate (PC), polyvinylchloride (PVC), polystyrene and mixtures and copolymers of the monomers of such polymers”

.

5. A component according to one of claims 1 to 4, characterized by that the front faces of at least one end of the fiber elements, preferably of both ends, are optically contactable.

6. A component according to one of claims 1 to 5, characterized by that the fiber elements have a diameter in the range of 0.01 μ

m to 1,000 μ

m.

7. A component according to claim 1 to 6, characterized by that the fiber elements have a length in the range of 0.1 mm to 100 mm.

8. A component according to one of claims 1 to 7, characterized by that the fiber elements are packed in a density of 1 to 10⁷ fibers/cm², referred to a radial cross-section plane of the fiber elements.

9. A component according to one of claims 1 to 8, characterized by that the supporting element is arranged at one end of the fiber elements and structured so to enclose the ends of the fiber elements, the front faces of the enclosed fiber elements being directly or indirectly optically contactable.

10. A component according to one of claims 1 to 9, characterized by that one supporting element each is arranged at both ends of the fiber elements.

11. A component according to one of claims 1 to 8, characterized by that the supporting element is arranged between the two ends of the fiber elements, for instance centrally.

12. A component according to one of claims 1 to 11, characterized by that the supporting element is adapted as a perforated plate.

13. A component according to one of claims 1 to 12, characterized by that the supporting element is configured as a wound-up supporting ribbon.

14. A component according to one of claims 1 to 13, characterized by that the sample molecule species or sample molecule species is selected from the group consisting of “

nucleic acids, DNA, RNA, PNA, aptamers, proteins, peptides, saccharides and mixtures of these sample molecules”

.

15. A component according to one of claims 1 to 14, characterized by that each fiber element carries sample molecules of a respectively selected, different sample molecule species.

16. A component according to one of claims 1 to 15, characterized by that each fiber element carries sample molecules of a respectively selected, different sample molecule species group, the group elements of every sample molecule species group commonly binding under generation of co-operative effects to a defined target molecule.

18. A component according to one of claims 4 to 17, characterized by that the polymeric material does not carry any functional groups at its surface, that the sample molecules are nucleic acids, and that the nucleic acids from an aqueous fixing solution containing MgCl₂ and NaCl are bound under irradiation with UV light to the surface of the polymeric material.

19. A method for the production of a component according to one of claims 1 to 18, comprising the following steps:

a)at least one endless fiber is produced,

b)the endless fiber is guided through a fluid containing a selected sample molecule species or a selected sample molecule species group,

c) the sample molecules of the sample molecule species or sample molecule species group are immobilized on the endless fiber,

d) as an option the endless fiber is supplied to at least one washing step,

e) to the endless fiber is associated the sample molecule species or sample molecule species group immobilized on the fiber in step c)

f)from different endless fibers or from different regions of an endless fiber, one fiber element each is cut off, and the fiber elements of various endless fibers or sections are bundled and fixed.

20. The use of a component according to one of claims 1 to 18 in a method for the detection of target molecules, wherein optically contactable front faces of the fiber elements are optically connected to a detector being sensitive to optical radiation of the wavelength to be detected, and wherein signals of the detector are respectively associated to the fiber elements, comprising the following steps:

a) to the component is supplied a solution with prospective target molecules, under conditions at which target molecules bind to sample molecules,

b) simultaneously with step a) or subsequently thereto the component is irradiated with a primary radiation exciting a wavelength to be detected,

c)simultaneously with step b) or subsequently thereto a reading-out of the signals of the detector and processing and storage of the signals is performed.

21. The use of a component according to one of claims 1 to 18 in a method for the preparative processing of a solution containing a mixture of substances, the component carrying sample molecules binding to target molecules to be separated from the mixture of substances, comprising the following steps:

a) the solution is supplied to the component, the target molecules to be separated being bound to sample molecules and being thus immobilized,

b)then the solution made free from target molecules to be separated is led away from the component and possibly supplied to another processing step, for instance to a method according to one of claims 26 or 27.

17. A component according to claim 17, characterized by that each sample molecule species group contains two group elements.