Micro-lasing beads and structures for combinatorial chemistry and other applications, and techniques for fabricating the structures and for detecting information encoded by the structures

• US 20030162296A1
• Filed: 02/05/2003
• Published: 08/28/2003
• Est. Priority Date: 05/13/1998
• Status: Abandoned Application

• Current Assignee: Nabil M. Lawandy
• Original Assignee: Nabil M. Lawandy
• Inventors: Lawandy, Nabil M.
• Application Number:
  US10/358,609
• Publication Number:
  US 20030162296A1
• Time in Patent Office: Days
• US Class Current: 436/56
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00457 Dispensing or evacuation of the solid phase support
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00565 Electromagnetic means
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00646 the compounds being bound to beads immobilised on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: H01S 3/06 Construction or shape of active medium
• CPC Class Codes: H01S 3/067 Fibre lasers
• CPC Class Codes: H01S 3/08086 Multiple-wavelength emission
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

An elongated structure includes a core, at least one and preferably a plurality of gain medium layers disposed about said core for providing a plurality of characteristic emission wavelengths, and a growth matrix or functionalized support suitable for the synthesis therein or thereon of a chemical compound. Other embodiments can be spherical, or planar with a plurality of optical gain medium dots, each providing a different emission wavelengths. Also disclosed is a technique for selectively locating micro-laser beads of interest, and then aiming a laser source at the bead(s) of interest in order to interrogate the optically encoded identification information. Also disclosed is a bead of a type that includes a functionalized support, and that further includes a gain medium coupled to a structure that supports the creation of at least one mode for electromagnetic radiation, and/or which has a dimension or length in one or more directions for producing and supporting amplified spontaneous emission (ASE). The structure can have boundaries that impart an overall geometry to the structure that, in combination with at least one material property of the structure, supports an enhancement of electromagnetic radiation emitted from the gain medium by favoring the creation of at least one mode that enhances an emission of electromagnetic radiation within a narrow band of wavelengths.

Claims

1. A structure, comprising:

a core;

at least one gain medium layer disposed about said core for providing a characteristic emission wavelength; and

a functionalized support for attaching to a desired substance.

2. A structure, comprising:

a core;

a plurality of gain medium layers disposed about said core for providing a plurality of characteristic emission wavelengths, said plurality of gain medium layers being adjacent to isolation layers having a larger index of refraction; and

a functionalized support for attaching to a desired substance.

3. A multispectral source of light comprising at least one pump laser, means for selectively providing at least one pump wavelength to a plurality of optical channels that comprise at least one Raman-based resonator structure for generating at least one of Red and Blue light, and for illuminating at least one micro-laser bead structure that comprises a functionalized support for attaching to a desired substance.

4. A light source as in claim 3, wherein the plurality of optical channels are a Red channel, a Green channel, and a Blue channel.

5. A light source as in claim 3, wherein the outputs of the plurality of optical channels are provided for exciting the bead structure to emit an identifying set of wavelengths.

6. A light source as in claim 5, and further comprising a spectrometer for resolving and detecting said emitted set of wavelengths.

7. A light source as in claim 6, and further comprising means for identifying an individual bead structure in accordance with the detected set of emitted wavelengths.

8. A method for fabricating a laser bead structure, comprising steps of:

providing a substrate;

depositing a plurality of regions of optical gain material on a surface of said substrate, each region being comprised of a plurality of areas each containing optical gain material, each area being capable of emitting a predetermined wavelength that differs from a wavelength emitted by others of said plurality of areas within said region; and

physically dividing the substrate into a plurality of individual laser bead structures individual ones of which comprise at least one of said areas.

9. A method as in claim 8, wherein the step of depositing employs a head structure for selectively printing optical gain material into said areas, and a mechanism for causing relative motion between the head and the substrate.

10. A method as in claim 8, wherein the step of depositing deposits a full complement of optical gain material into said plurality of areas, and further comprising a step of selectively removing or deactivating optical gain material within selected ones of said areas.

11. A method as in claim 10, wherein the step of selectively removing comprises a step of photo-bleaching the optical gain material in selected ones of said areas.

12. A method as in claim 10, wherein the step of selectively removing comprises a step of photo-ablating the optical gain material in selected ones of said areas.

13. A structure, comprising:

a substrate;

a plurality of areas on a surface of said substrate, each of said areas comprising an optical gain medium material capable of emitting a predetermined wavelength that differs from a wavelength emitted by others of said plurality of areas; and

a functionalized support for attaching to a desired substance.

14. A structure as in claim 13, and further comprising a protective transparent substrate disposed between said surface and the environment.

15. A method for identifying a particular bead in a population of beads, comprising steps of:

providing a population of beads each comprising a functionalized support and means for optically encoding identification information;

using a sensor that is responsive to a desired bead activity for identifying a location of one or more beads of interest within the population;

using the identified location to aim an interrogation beam at a particular bead; and

determining an identification of the particular bead from a plurality of wavelengths emitted by the particular bead in response to the interrogation beam.

16. A method as in claim 15, wherein the sensor is comprised of at least one of an optical energy detector, an ionizing radiation detector, or a thermal energy detector.

17. A method as in claim 15, wherein the sensor is capable of operating with more than one sensitivity threshold.

18. A bead comprising a functionalized support and further comprising a gain medium coupled to a structure that supports the creation of at least one mode for electromagnetic radiation.

19. A bead comprising a functionalized support and further comprising a gain medium coupled to a structure having a dimension or length in one or more directions for producing and supporting amplified spontaneous emission (ASE).

20. A bead comprising a functionalized support and further comprising an optical gain medium and a structure having boundaries that impart an overall geometry to said structure that, in combination with at least one material property of said structure, supports an enhancement of electromagnetic radiation emitted from the gain medium by favoring the creation of at least one mode that enhances an emission of electromagnetic radiation within a narrow band of wavelengths.

21. A bead as in claim 20, wherein suitable shapes for said structure comprise elongated, generally cylindrical shapes such as filaments, a spherical shape, a partial-spherical shape, a toroidal shape, a cubical and other polyhedral shape, and a disk shape.

22. A bead as in claim 20, wherein said structure is comprised of at least one of a monolithic structure or a multi-layered structure or an ordered structure that may provide for distributed optical feedback for the creation of a mode.

23. A method for identifying a bead of a type that comprises a functionalized support, comprising the steps of:

providing the bead so as to comprise an optical gain medium and a structure for at least one of (a) favoring the creation of at least one mode or (b) supporting amplified spontaneous emission;

illuminating the bead with light selected for exciting the gain medium;

detecting an emission of at least one wavelength from the bead in response to the step of illuminating; and

identifying the bead from the detected emission.

24. A method as in claim 23, wherein step of providing provides at least one of a polymer layer that functions as the structure that favors the creation of the at least one mode;

at least one filament;

a multilayered structure;

a multilayered structure that is comprised of a reflecting layer; and

a multilayered structure comprised of a reflecting layer that is patterned and that modulates a thickness of an overlying layer.

25. A method as in claim 23, wherein the structure has an index of refraction that differs from an index of refraction of an environment of the structure such that the structure is non-indexed matched to the environment.

26. A method as in claim 23, wherein the structure is comprised of at least one filament, and wherein the emitted wavelength is a function of a diameter of the filament.

27. A method as in claim 23, wherein the structure is comprised of a planchette, and wherein the emitted wavelength is a function of the thickness of the planchette.

28. A method as in claim 23, wherein the structure is comprised of a DFB structure comprised of alternating regions, and wherein the emitted wavelength is a function of the thickness of individual ones of the regions.

29. A method for processing a population of beads of a type that comprise a functionalized support, comprising the steps of:

providing at least some beads of the population so as to comprise an optical gain medium and a structure coupled to said gain medium for at least one of (a) favoring the creation of at least one mode or (b) supporting amplified spontaneous emission, said structure encoding information that is made manifest by an optical emission from said bead;

illuminating at least a portion of the population with light selected for exciting the gain medium;

detecting an emission of at least one wavelength from at least one bead in response to the step of illuminating; and

decoding the information that was encoded in the at least one bead from the detected emission.

30. A method as in claim 29, wherein the information is encoded using only wavelength encoding or both wavelength encoding and signal level encoding.

31. A method as in claim 29, wherein the information is encoded using at least one of single level encoding or multi-level encoding.

32. A method for identifying a particular bead in a population of beads in one of a combinatorial chemistry, a screening, or a genomic application, comprising steps of:

providing a population of beads each comprising a functionalized support and means for optically encoding identification information;

using a sensor that is responsive to a desired bead activity for identifying a location of one or more beads of interest within the population, said sensor being comprised of at least one of an optical energy detector, an ionizing radiation detector, or a thermal energy detector;

using the identified location to aim an interrogation laser beam at a particular bead; and

determining an identification of the particular bead from a plurality of wavelengths emitted by the particular bead in response to the interrogation laser beam.

33. A method as in claim 32, wherein the sensor located within or beneath a container that holds the population of beads.

34. A method for identifying a particular bead in a population of beads used in a Lawn Assay, comprising steps of:

providing a population of beads each comprising a functionalized support and means for optically encoding identification information;

using a sensor detects bead assay activity for identifying a location of one or more beads of interest within the population, said sensor being comprised of at least one of an optical energy detector, an ionizing radiation detector, or a thermal energy detector;

using the identified location to aim an interrogation laser beam at a particular bead; and

determining an identification of the particular bead from a plurality of wavelengths emitted by the particular bead in response to the interrogation laser beam.

35. A method as in claim 34, wherein the sensor is located within or beneath a container that holds the population of beads.