Orthogonal read assembly

• US 20030162308A1
• Filed: 12/04/2002
• Published: 08/28/2003
• Est. Priority Date: 12/04/2001
• Status: Abandoned Application

• Current Assignee: Dave Smith, Eric Waltzer, Greg Liang, Mike Smith, Roger Tatum, Thomas J. Foley
• Original Assignee: Dave Smith, Eric Waltzer, Greg Liang, Mike Smith, Roger Tatum, Thomas J. Foley
• Inventors: Liang, Greg, Smith, Dave, Smith, Mike, Waltzer, Eric, Tatum, Roger, Foley, Thomas J.
• Application Number:
  US10/310,680
• Publication Number:
  US 20030162308A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: A61B 10/0051 for taking saliva or sputum samples devices for receiving spittle A61J9/00
• CPC Class Codes: A61B 10/0283 with vacuum aspiration, e.g. caused by retractable plunger or by connected syringe A61B10/025, A61B10/0266 take precedence
• CPC Class Codes: B01F 2101/23 Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
• CPC Class Codes: B01F 33/452 using independent floating stirring elements
• CPC Class Codes: B01F 33/81 Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
• CPC Class Codes: B01F 33/812 in two or more alternative mixing receptacles, e.g. mixing in one receptacle and dispensing from another receptacle
• CPC Class Codes: B01F 35/713 comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges containers or packages per se B65D
• CPC Class Codes: B01F 35/7137 Piercing, perforating or melting membranes or closures which seal the compartments
• CPC Class Codes: B01F 35/7161 the containers being connected coaxially before contacting the contents
• CPC Class Codes: B01F 35/7163 the containers being connected in a mouth-to-mouth, end-to-end disposition, i.e. the openings are juxtaposed before contacting the contents
• CPC Class Codes: B01F 35/7174 using pistons, plungers or syringes
• CPC Class Codes: B01F 35/754251 reciprocating in the mixing receptacle
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/5023 : with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: G01N 1/02 : Devices for withdrawing samples sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 33/48714 : for determining substances foreign to the organism, e.g. drugs or heavy metals drugs by chemical analysis G01N33/94
• CPC Class Codes: G01N 33/54313 : the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54373 : involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/98 : involving alcohol, e.g. ethanol in breath
• CPC Class Codes: Y10T 137/7036 : Jacketed
• CPC Class Codes: Y10T 156/1002 : with permanent bending or reshaping or surface deformation of self sustaining lamina
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Methods and assemblies are provided for transmitting and detecting energy within a read cell. An orthogonal read assembly includes an immunoassay reaction chamber, a read cell having a lumen in fluid communication with the immunoassay reaction chamber, a transmitter configured to transmit energy through the lumen, and a detector configured to sense energy emitted transversely from the lumen. The transmitter can include an optical transmitter, such as a laser, and the detector can include an optical detector, e.g., a silicon diode. The optical transmitter can transmit optical energy at an oblique entry angle to the lumen, e.g., 45°, and the optical energy can be sensed by the optical detector at an angle substantially perpendicular to the lumen. The read cell can be composed of a transparent plastic and can be parallel-pipe shaped. The lumen can be cylindrically shaped and may include an optical transmission port, in which case, the optical transmitter can be configured to transmit the optical energy through the lumen via the optical transmission port.

Claims

1. An orthogonal read assembly, comprising:

an immunoassay reaction chamber;

a read cell comprising a lumen in fluid communication with said immunoassay reaction chamber;

a transmitter configured to transmit energy through said lumen; and

a detector configured to sense energy emitted transversely from said lumen.

2. The orthogonal read assembly of claim 1, wherein said transmitter transmits said energy at an oblique entry angle to said lumen.

3. The orthogonal read assembly of claim 2, wherein said oblique angle is substantially 45°

.

4. The orthogonal read assembly of claim 1, wherein said transmitter comprises an optical transmitter, and said detector comprises an optical detector.

5. The orthogonal read assembly of claim 1, wherein said transmitter comprises a laser, and said detector comprises an optical detector.

6. The orthogonal read assembly of claim 5, wherein said laser is configured to transmit laser energy at a wavelength of approximately 655 nm, and said optical detector is configured to sense optical energy at a wavelength of between 635 nm to 670 nm.

7. The orthogonal read assembly of claim 5, wherein said optical transmitter comprises a silicon diode.

8. The orthogonal read assembly of claim 1, wherein said energy is sensed from said lumen at an angle substantially perpendicular to said lumen.

9. The orthogonal read assembly of claim 1, wherein said lumen is cylindrically shaped.

10. The orthogonal read assembly of claim 1, wherein said read cell is composed of transparent plastic.

11. The orthogonal read assembly of claim 1, wherein said read cell is parallel-pipe shaped.

12. The orthogonal read assembly of claim 1, wherein said lumen comprises an energy transmission port, and said transmitter is configured to transmit said energy through said lumen via said transmission port.

13. The orthogonal read assembly of claim 1, wherein said energy transmission port comprises an optical energy transmission port, and said transmitter comprises an optical transmitter.

14. An orthogonal read assembly, comprising:

an immunoassay reaction chamber containing labeled antigen that is displaced when an analog to said labeled antigen flows through said immunoassay reaction chamber;

a read cell comprising a lumen in fluid communication with said immunoassay reaction chamber;

a transmitter configured to transmit energy through said lumen to excite said labeled antigen to transversely emit energy from said lumen; and

a detector configured to sense said transversely emitted energy from said labeled antigen.

15. The orthogonal read assembly of claim 14, wherein said transmitter transmits said energy at an oblique entry angle to said lumen.

16. The orthogonal read assembly of claim 15, wherein said oblique angle is substantially 45°

.

17. The orthogonal read assembly of claim 14, wherein said transmitter comprises an optical transmitter, said transmitted energy comprises optical energy, said detector comprises an optical detector, and said sensed energy comprises optical energy.

18. The orthogonal read assembly of claim 14, wherein said transmitter comprises a laser, said transmitted energy comprises laser energy, said detector comprises an optical detector, and said sensed energy comprises optical energy.

19. The orthogonal read assembly of claim 18, wherein said laser is configured to transmit laser energy at a wavelength of approximately 655 nm, and said optical detector is configured to sense optical energy at a wavelength of between 635 nm to 670 nm.

20. The orthogonal read assembly of claim 18, wherein said optical transmitter comprises a silicon diode.

21. The orthogonal read assembly of claim 14, wherein said labeled antigen comprises a fluorescent labeled antigen, said transmitter comprises a laser, said transmitted energy comprises laser energy that causes said fluorescent labeled antigen to fluoresce, said detector comprises an optical detector, and said sensed energy comprises fluorescent energy.

22. The orthogonal read assembly of claim 14, wherein said energy is sensed from said lumen at an angle substantially perpendicular to said lumen.

23. The orthogonal read assembly of claim 14, wherein said lumen is cylindrically shaped.

24. The orthogonal read assembly of claim 14, wherein said read cell is composed of transparent plastic.

25. The orthogonal read assembly of claim 14, wherein said read cell is parallel-pipe shaped.

26. The orthogonal read assembly of claim 14, wherein said lumen comprises a transmission port, and said transmitter is configured to transmit said energy through said lumen via said transmission port.

27. A method of sensing an analyte within a sample, comprising:

flowing said sample through an immunoassay reaction chamber to displace labeled antigen from said immunoassay reaction chamber;

flowing said displaced labeled antigen through a lumen of a read cell;

transmitting energy along a longitudinal axis of said lumen to excite said displaced labeled antigen into transversely emitting energy from said lumen; and

sensing said transversely emitted energy.

28. The method of claim 27, wherein said energy is transmitted at an oblique entry angle to said longitudinal axis.

29. The method of claim 28, wherein said oblique angle is a substantially 45°

angle.

30. The method of claim 27, wherein said transmitted energy comprises optical energy, and said transversely emitted energy comprises optical energy.

31. The method of claim 30, wherein said optical energy comprises laser energy.

32. The method of claim 31, wherein said laser energy has a wavelength of approximately 655 nm, and said transversely emitted energy has a wavelength of between 635 nm to 670 nm.

33. The method of claim 27, wherein said labeled antigen comprises a fluorescent labeled antigen, said transmitted energy comprises laser energy that causes said fluorescent labeled antigen to fluoresce, and said sensed energy comprises fluorescent energy.

34. The method of claim 27, wherein said transversely emitted energy is sensed from said lumen at an angle substantially perpendicular to said longitudinal axis.

35. The method of claim 27, wherein said lumen comprises a transmission port, and said energy is transmitted through said lumen via said transmission port.