Elongase genes and uses thereof
First Claim
1. An isolated nucleotide sequence or fragment thereof comprising or complementary to a nucleotide sequence encoding a polypeptide having elongase activity, wherein the amino acid sequence of said polypeptide has at least 50% identity to an amino acid sequence comprising SEQ ID NO:
- 121.
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Abstract
The subject invention relates to the identification of several genes involved in the elongation of polyunsaturated acids (i.e., “elongases”) and to uses thereof. At least two of these genes are also involved in the elongation of monounsaturated fatty acids. In particular, elongase is utilized in the conversion of gamma linolenic acid (GLA) to dihomogamma linolenic acid (DGLA) and in the conversion of AA to adrenic acid (ADA), or eicosapentaenoic acid (EPA) to ω3-docosapentaenoic acid (DPA). DGLA may be utilized in the production of polyunsaturated fatty acids, such as arachidonic acid (AA), docosahexaenoic acid (DHA), EPA, adrenic acid, ω6-docosapentaenoic acid or ω3-docosapentaenoic acid which may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
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Citations
42 Claims
- 1. An isolated nucleotide sequence or fragment thereof comprising or complementary to a nucleotide sequence encoding a polypeptide having elongase activity, wherein the amino acid sequence of said polypeptide has at least 50% identity to an amino acid sequence comprising SEQ ID NO:
- 2. An isolated nucleotide sequence or fragment thereof comprising or complementary to a nucleotide sequence having at least 50% nucleotide sequence identity to a nucleotide sequence comprising SEQ ID NO:
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8. A method of producing an elongase enzyme comprising the steps of:
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a) isolating a nucleotide sequence comprising SEQ ID NO;
119;
b) constructing a vector comprising;
i) said isolated nucleotide sequence operably linked to ii) a promoter;
c) introducing said vector into a host cell under time and conditions sufficient for expression of said elongase enzyme. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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15. A vector comprising:
- a) a nucleotide sequence comprising SEQ ID NO;
119 operably linked to b) a promoter. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
- a) a nucleotide sequence comprising SEQ ID NO;
- 27. A transgenic, non-human mammal whose genome comprises a DNA sequence encoding an elongase, operably linked to a promoter, wherein said DNA sequence comprises SEQ ID NO:
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29. A method for producing a polyunsaturated fatty acid comprising the steps of:
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a) isolating a nucleotide sequence comprising SEQ ID NO;
119;
b) constructing a vector comprising said isolated nucleotide sequence;
c) introducing said vector into a host cell under time and conditions sufficient for expression of an elongase enzyme encoded by said isolated nucleotide sequence; and
d) exposing said expressed elongase enzyme to a substrate polyunsaturated fatty acid in order to convert said substrate to a product polyunsaturated fatty acid. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 41, 42)
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Specification