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Use of phosphorothiolate polynucleotides in ligating nucleic acids

  • US 20030165841A1
  • Filed: 06/15/2001
  • Published: 09/04/2003
  • Est. Priority Date: 05/10/2001
  • Status: Active Grant
First Claim
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1. A method of non-enzymatic ligation of a nucleic acid, comprising contacting a polynucleotide-3′

  • phosphorothiolate with an acceptor polynucleotide under conditions that allow formation of a phosphodiester bond between said polynucleotide-3′

    phosphorothiolate and said acceptor polynucleotide.

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