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Primers and methods for the detection and discrimination of nucleic acids

  • US 20030165859A1
  • Filed: 12/27/2001
  • Published: 09/04/2003
  • Est. Priority Date: 10/23/2001
  • Status: Abandoned Application
First Claim
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1. A composition for quantifying or detecting one or more target nucleic acid molecules in a sample, comprising at least three oligonucleotides, wherein the first oligonucleotide has a tail at the 5′

  • -end of the gene-specific target primer which is non-complementary to the target sequence and which is identical to the 3′

    -end sequence of the second oligonucleotide;

    the second oligonucleotide is at least partially identical to the tail of the first oligonucleotide and is labeled with a fluorescent moiety; and

    the third oligonucleotide is a primer complementary to the 3′

    -end of the target.

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