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Detection of bordetella

  • US 20030165866A1
  • Filed: 01/31/2002
  • Published: 09/04/2003
  • Est. Priority Date: 01/31/2001
  • Status: Abandoned Application
First Claim
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1. A method for detecting the presence or absence of Bordetella pertussis in a biological sample from an individual, said method comprising:

  • performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of IS481 primers to produce an IS481 amplification product if a B. pertussis IS481 nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of IS481 probes, wherein the members of said pair of IS481 probes hybridize within no more than five nucleotides of each other, wherein a first IS481 probe of said pair of IS481 probes is labeled with a donor fluorescent moiety and a second IS481 probe of said pair of IS481 probes is labeled with a corresponding acceptor fluorescent moiety; and

    detecting the presence or absence of fluorescence resonance energy transfer (FRET) between said donor fluorescent moiety of said first IS481 probe and said corresponding acceptor fluorescent moiety of said second IS481 probe, wherein the presence of FRET is indicative of the presence of B. pertussis in said biological sample, and wherein the absence of FRET is indicative of the absence of B. pertussis in said biological sample.

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