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Multi-test analysis of real-time nucleic acid amplification

  • US 20030165867A1
  • Filed: 02/12/2002
  • Published: 09/04/2003
  • Est. Priority Date: 02/12/2002
  • Status: Active Grant
First Claim
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1. A method of determining the presence of a nucleic acid in a sample comprising the steps of providing a fluorescent entity capable of indicating the presence of the nucleic acid and capable of providing a signal related to the quantity of the nucleic acid, amplifying the nucleic acid through a plurality of amplification cycles in the presence of the fluorescent entity, measuring fluorescence intensity of the fluorescent entity at each of the plurality of amplification cycles to produce a fluorescent value for each cycle related to the quantity of the nucleic acid present at each cycle, obtaining an individual score from each of a plurality of tests, the plurality of tests comprising a Confidence Interval Test and a Signal-to-Noise Ratio Test, and using the scores to ascertain whether the nucleic acid is present in the sample.

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