Human glycine transporter type 2
First Claim
Patent Images
1. A purified nucleic acid comprising a nucleic acid sequence that encodes a glycine transporter type 2 (GlyT2) amino acid sequence that has glycine transport activity and that has a serine at a position corresponding to amino acid 24 of SEQ ID NO:
- 124.
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Abstract
Nucleic acids and proteins derived from sequences of the human glycine transporter type 2 are described.
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Citations
82 Claims
- 1. A purified nucleic acid comprising a nucleic acid sequence that encodes a glycine transporter type 2 (GlyT2) amino acid sequence that has glycine transport activity and that has a serine at a position corresponding to amino acid 24 of SEQ ID NO:
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2. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has a tryptophan at a position corresponding to amino acid 74 of SEQ ID NO:
- 124.
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3. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has a glycine at a position corresponding to amino acid 155 of SEQ ID NO:
- 124.
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4. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has an aspartic acid at a position corresponding to amino acid 188 of SEQ ID NO:
- 124.
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5. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has a leucine at a position corresponding to amino acid 362 of SEQ ID NO:
- 124.
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6. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has an alanine at a position corresponding to amino acid 431 of SEQ ID NO:
- 124.
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7. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has a serine at a position corresponding to amino acid 582 of SEQ ID NO:
- 124.
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8. A purified nucleic acid comprising a nucleic acid sequence that encodes a GlyT2 amino acid sequence that has glycine transport activity and that has one or more of the following amino acids:
- (1) serine at a position corresponding to amino acid 24, (2) tryptophan at a position corresponding to amino acid 74, (3) glycine at a position corresponding to amino acid 155, (4) aspartic acid at a position corresponding to amino acid 188, (5) leucine at a position corresponding to amino acid 362, (6) alanine at a position corresponding to amino acid 431, or (7) serine at a position corresponding to amino acid 582, of SEQ ID NO;
124.
- (1) serine at a position corresponding to amino acid 24, (2) tryptophan at a position corresponding to amino acid 74, (3) glycine at a position corresponding to amino acid 155, (4) aspartic acid at a position corresponding to amino acid 188, (5) leucine at a position corresponding to amino acid 362, (6) alanine at a position corresponding to amino acid 431, or (7) serine at a position corresponding to amino acid 582, of SEQ ID NO;
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47. A purified nucleic acid encoding a glycine transporter type 2 (GlyT2) having at least about 96% sequence identity with a reference sequence which is the protein sequence of SEQ ID NO:
- 122 or with a sequence corresponding to the protein sequence of SEQ ID NO;
122 except that it has one or more of the following amino acid substitutions;
(1) Pro26 to Leu, (2) Arg74 to Trp, (3) Pro75 to Leu, (4) Ala89 to Val, (5) Ser102 to Gly, (6) Val174 to Glu, (7) Ser195 to Pro, (8) Asp199 to Gly, (9) Val249 to Leu, (10) Leu306 to Pro, (11) Gly419 to Glu, (12) Thr442 to Asn, (13) Thr455 to Lys, (14) Trp458 to Cys, (15) Leu485 to Pro, (16) Lys493 to Arg, or (17) Val650 to Glu. - View Dependent Claims (48, 49, 50, 51, 52, 54, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 81, 82)
- 122 or with a sequence corresponding to the protein sequence of SEQ ID NO;
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53. The nucleic acid sequence of SEQ ID NO:
- 121 or a sequence that varies from the nucleic acid sequence of SEQ ID NO;
121 by having one or more of the following nucleotide substitutions;
C220→
T, A304→
G, or T1292→
C.
- 121 or a sequence that varies from the nucleic acid sequence of SEQ ID NO;
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55. A nucleic acid encoding a glycine transporter protein having at least about 99.5% sequence identity with all or one to two contiguous portions of a reference amino acid sequence, wherein the reference sequence is SEQ ID NO:
- 122 or an amino acid sequence corresponding to the amino acid sequence of SEQ ID NO;
122 except that it has one or more of the following amino acid substitutions;
(1) Pro26 to Leu, (2) Arg74 to Trp, (3) Pro75 to Leu, (4) Ala89 to Val, (5) Ser102 to Gly, (6) Val174 to Glu, (7) Ser195 to Pro, (8) Asp199 to Gly, (9) Val249 to Leu, (10) Leu306 to Pro, (11) Gly419 to Glu, (12) Thr442 to Asn, (13) Thr455 to Lys, (14) Trp458 to Cys, (15) Leu485 to Pro, (16) Lys493 to Arg, or (17) Val650 to Glu. - View Dependent Claims (56)
- 122 or an amino acid sequence corresponding to the amino acid sequence of SEQ ID NO;
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73. A nucleic acid that hybridizes with a reference nucleic acid sequence which is SEQ ID NO:
- 121 or with a sequence that varies from the nucleic acid sequence of SEQ ID NO;
121 by having one or more of the following nucleotide substitutions;
(a) C77→
T, (b) C220→
T, (c) C224→
T, (d) C266→
T, (e) A304→
G, (f) T521→
A, (g) T583→
C, (h) A596→
G, (i) A678→
G, (j) T681→
C, (k) G745→
T, (l) G750→
C, (m) C765→
T, (n) G777→
C, (o) A867→
G, (p) T917→
C, (q) G1256→
A, (r) T1292→
C, (s) C1325→
A, (t) C1364→
A, (u) G1374→
C, (v) C1392→
A, (w) T1454→
C, (x) A1478→
G, (y) T1854→
C, (z) T1949→
A, (aa) T1959→
C, or (bb) T2130→
C, under conditions of sufficient stringency to exclude hybridizations with (a) the sequence for a rat GlyT2 transporter or (b) the sequence for a mammalian GlyT1 transporter. - View Dependent Claims (74, 76, 77, 78, 79)
- 121 or with a sequence that varies from the nucleic acid sequence of SEQ ID NO;
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75. A nucleic acid of at least about eighteen nucleotides in length comprising a contiguous sequence from the coding or non-coding strand of a human GlyT2 gene or cDNA, wherein the contiguous sequence has at least 1 nucleotide difference when compared with the rat GlyT2 gene sequence that aligns with the contiguous sequence.
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80. A method for detecting whether an animal has autoimmune antibodies against a glycine transporter, the method comprising contacting an antibody preparation from the animal or a body fluid from the animal with a polypeptide antigen comprising a glycine transporter or derived from the glycine transporter.
Specification