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Method for identification of genetic markers

  • US 20030165923A1
  • Filed: 09/06/2002
  • Published: 09/04/2003
  • Est. Priority Date: 06/30/2000
  • Status: Active Grant
First Claim
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1. A method for identifying genetic markers comprising:

  • a) obtaining genomic DNA from a first source having a first phenotype or trait of interest;

    b) digesting said DNA with at least one restriction endonuclease having a degenerate recognition or cleavage sequence comprising at least one degenerate base said degenerate sequence represented by the formula Nm, where N is the extent of degeneracy, and m is the number of degenerate bases, to produce restriction fragments having Nm different single-stranded overhangs for each restriction endonuclease;

    c) ligating restriction fragments having identical overhangs with a series of adapters whose sequences are complementary to said overhangs;

    d) amplifying said restriction fragments;

    e) separating said amplified restriction fragments to produce a restriction fragment pattern;

    f) obtaining genomic DNA from at least one additional source having a second phenotype or trait of interest;

    g) repeating b) through e) for said DNA from said at least one additional source;

    h) comparing the restriction fragment patterns from each source;

    i) selecting restriction fragments associated with a phenotype or trait of interest; and

    j) using said selected restriction fragments as markers for said phenotype or trait of interest.

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