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Novel methods for amplifying and detecting nucleic acid sequences

  • US 20030165938A1
  • Filed: 11/29/2002
  • Published: 09/04/2003
  • Est. Priority Date: 06/24/1998
  • Status: Abandoned Application
First Claim
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1. A process for linearly amplifying a specific nucleic acid sequence comprising the steps of:

  • providing;

    said specific nucleic acid sequence, an initial primer or a nucleic acid construct comprising two segments, (A) a first segment (i) being substantially complementary to a first portion of said specific nucleic acid sequence and (ii) capable of template-dependent first extension, and (B) a second segment being (i) substantially non-identical to said first segment, (ii) substantially identical to a second portion of said specific nucleic acid sequence, (iii) capable of binding to a complementary sequence of said second segment and (iv) capable of providing for subsequent binding of a first segment of a second primer or nucleic acid construct to said first portion of said specific nucleic acid sequence under isostatic or limited cycling conditions, such that a second primer extension is produced and displaces a first primer extension; and

    substrates, buffer and a template-dependent polymerizing enzyme; and

    incubating said specific nucleic acid sequence and said novel primer or nucleic acid construct in the presence of said substrates, buffer and template-dependent polymerizing enzyme under isostatic or limited cycling conditions;

    thereby linearly amplifying said specific nucleic acid sequence.

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