Reagents and methods useful for detecting diseases of the breast
First Claim
1. A method of detecting the presence of a target BS106 polynucleotide in a test sample, comprising:
- (a) contacting said test sample with at least one BS106-specific polynucleotide or complement thereof; and
(b) detecting the presence of said target BS106 polynucleotide in the test sample, wherein said BS106-specific polynucleotide has at least 50% identity to a polynucleotide selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof.
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Abstract
A set of contiguous and partially overlapping RNA sequences and polypeptides encoded thereby, designated as BS106 and transcribed from breast tissue is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, preventing or treating, or determining the, predisposition of an individual to diseases and conditions of the breast such as breast cancer. Also provided are antibodies which specifically bind to BS106-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific BS106 polypeptide, which molecules are useful for the therapeutic treatment of breast diseases, tumors or metastases.
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Citations
39 Claims
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1. A method of detecting the presence of a target BS106 polynucleotide in a test sample, comprising:
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(a) contacting said test sample with at least one BS106-specific polynucleotide or complement thereof; and
(b) detecting the presence of said target BS106 polynucleotide in the test sample, wherein said BS106-specific polynucleotide has at least 50% identity to a polynucleotide selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof. - View Dependent Claims (2)
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3. A method for detecting mRNA of BS106 in a test sample, comprising:
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(a) performing reverse transcription with at least one primer in order to produce cDNA;
(b) amplifying the cDNA obtained from step (a) using BS106 oligonucleotides as sense and antisense primers to obtain BS106 amplicon; and
(c) detecting the presence of said BS106 amplicon in the test sample, wherein the BS106 oligonucleotides utilized in steps (a) and (b) have at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof. - View Dependent Claims (4, 5)
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6. A method of detecting a target BS106 polynucleotide in a test sample suspected of containing said target, comprising:
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(a) contacting said test sample with at least one BS106 oligonucleotide as a sense primer and with at least one BS106 oligonucleotide as an anti-sense primer and amplifying to obtain a first stage reaction product;
(b) contacting said first stage reaction product with at least one other BS106 oligonucleotide to obtain a second stage reaction product, with the proviso that the other BS106 oligonucleotide is located 3′
to the BS106 oligonucleotides utilized in step (a) and is complementary to said first stage reaction product; and
(c) detecting said second stage reaction product as an indication of the presence of the target BS106 polynucleotide, wherein the BS106 oligonucleotides utilized in steps (a) and (b) have at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof. - View Dependent Claims (7, 8, 9)
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- 10. A test kit useful for detecting BS106 polynucleotide in a test sample, comprising a container containing at least one BS106 polynucleotide having at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof.
- 11. A purified polynucleotide or fragment thereof derived from a BS106 gene, wherein said polynucleotide is capable of selectively hybridizing to the nucleic acid of said BS106 gene and has at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof.
- 15. A recombinant expression system comprising a nucleic acid sequence that includes an open reading frame derived from BS106 operably linked to a control sequence compatible with a desired host, wherein said nucleic acid sequence has at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof.
- 17. A BS106 polypeptide having at least 50% identity with an amino acid sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments of SEQUENCE ID NOS 17-20.
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20. An antibody which specifically binds to at least one BS106 epitope, wherein said BS106 epitope is derived from an amino acid sequence having at least 50% identity to an amino acid sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof.
- 21. An assay kit for determining the presence of BS106 antigen or anti-BS106 antibody in a test sample, comprising a container containing a BS106 polypeptide having at least 50% identity with an amino acid sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof.
- 23. An assay kit for determining the presence of BS106 antigen in a test sample, comprising a container containing an antibody which specifically binds to a BS106 antigen which comprises at least one BS106 epitope.
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25. A method for producing a polypeptide comprising at least one BS106 epitope, said method comprising incubating host cells that have been transfected with an expression vector containing a polynucleotide sequence encoding a polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 50% identity to an amino acid sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof.
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26. A method for detecting BS106 antigen in a test sample suspected of containing said BS106 antigen, comprising:
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(a) contacting the test sample with an antibody or fragment thereof which specifically binds to at least one epitope of a BS106 antigen selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof, wherein said contacting is carried out for a time and under conditions sufficient for the formation of antibody/antigen complexes; and
(b) detecting the presence of said complexes as an indication of the presence of said BS106 antigen. - View Dependent Claims (27)
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28. A method for detecting the presence of antibodies specific for a BS106 antigen in a test sample suspected of containing such antibodies, said method comprising:
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(a) contacting the test sample with a BS106 polypeptide, wherein said BS106 polypeptide contains at least one BS106 epitope derived from an amino acid sequence or fragment thereof having at least 50% identity to an amino acid sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof, and further wherein said contacting is carried out for a time and under conditions sufficient to allow antigen/antibody complexes to form; and
(b) detecting said complexes. - View Dependent Claims (29)
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30. A cell transfected with a nucleic acid sequence encoding at least one BS106 epitope, wherein said nucleic acid sequence is selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof.
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31. A method for producing antibodies which specifically bind to BS106 antigen, comprising administering to an individual an isolated immunogenic polypeptide or fragment thereof in an amount sufficient to elicit an immune response, wherein said immunogenic polypeptide comprises at least one BS106 epitope and has at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof.
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32. A method for producing antibodies which specifically bind to BS106 antigen, comprising administering to a mammal a plasmid comprising a sequence which encodes at least one BS106 epitope derived from a polypeptide having an amino acid sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments thereof.
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33. A composition of matter comprising a BS106 polynucleotide or fragment thereof, wherein said polynucleotide has at least 50% identity to a polynucleotide selected from the group consisting of SEQUENCE ID NO 1, SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, and fragments or complements thereof.
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34. A composition of matter comprising a polypeptide containing at least one BS106 epitope, wherein said polypeptide has at least 50% identity to a sequence selected from the group consisting of SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, and fragments of SEQUENCE ID NOS 17-20.
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38. A gene or fragment thereof which codes for a BS106 protein which comprises an amino acid sequence with at least 50% identity with SEQUENCE ID NO 16.
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39. A gene or fragment thereof comprising DNA having at least 50% identity with SEQUENCE ID NO 4 or SEQUENCE ID NO 5.
Specification