Compositions and methods for surface imprinting
First Claim
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1. A surface imprint composition comprising a matrix material defining imprint cavities of a template molecule wherein a substantial fraction of the imprint cavities are localized at or near the surface of the matrix material.
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Abstract
The present invention provides surface imprint compositions useful for capturing, isolating, detecting, analyzing and/or quantifying molecules in a sample. The surface imprint compositions comprise a matrix material having imprint cavities of a template molecule or molecules imprinted thereon wherein a- substantial number of the imprint cavities are located at or near the surface of the matrix material.
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Citations
57 Claims
- 1. A surface imprint composition comprising a matrix material defining imprint cavities of a template molecule wherein a substantial fraction of the imprint cavities are localized at or near the surface of the matrix material.
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27. A surface imprint composition comprising a matrix material defining imprint cavities of a template molecule wherein a substantial fraction of the imprint cavities are oriented.
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28. A method of preparing a surface imprint comprising the steps of:
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(a) forming a hardened matrix in the presence of an immobilized template molecule; and
(b) removing the template molecule from the hardened matrix, yielding a surface imprint. - View Dependent Claims (29, 30, 31, 32, 33, 34)
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35. A method of making a surface imprint comprising the steps of:
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(a) dispersing a polymerizable compound and a conjugate molecule in a solvent system which comprises a first solvent and a second solvent which is immiscible with the first solvent such that they form a two-phase system wherein the polymerizable compound and the template moiety of the conjugate molecule partition into the same phase of the two-phase system;
(b) polymerizing the polymerizable compound; and
(c) removing the conjugate molecule. - View Dependent Claims (36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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55. A method of making an surface imprint array capable of capturing a plurality of different molecules, comprising the steps of:
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(a) forming a hardened matrix in the presence of an array of immobilized template molecules; and
(b) removing at least two of the template molecules from the hardened matrix yielding a surface imprint array.
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56. A method of screening a plurality of macromolecules, comprising contacting the plurality of macromolecules with a matrix, said matrix comprising an surface imprint of a template molecule wherein the template molecule is selected from a peptide consisting of 3 to 30 amino acids, a polynucleotide consisting of 3 to 30 nucleotides, and an oligosaccharide consisting of 3 to 30 saccharides, under conditions in which at least one molecule of the plurality binds the matrix.
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57. A method of screening a plurality of macromolecules, comprising contacting the plurality of macromolecules with a plurality of matrices, said matrices comprising a plurality of surface imprints of template molecules, wherein at least two of the template molecules are unique, wherein the template molecules are selected from a peptide consisting of 3 to 30 amino acids, a polynucleotide consisting of 3 to 30 nucleotides, and an oligosaccharide consisting of 3 to 30 saccharides, and under conditions in which at least one molecule of the plurality binds a matrix.
Specification