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Method for accurately mixing sample and buffer solutions

  • US 20030166259A1
  • Filed: 12/04/2002
  • Published: 09/04/2003
  • Est. Priority Date: 12/04/2001
  • Status: Abandoned Application
First Claim
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1. A method of mixing a sample and buffer using a buffer chamber, sample collection chamber, and mixing chamber, said buffer and sample collection chambers being in fluid communication with said mixing chamber via respective buffer and sample ports, the method comprising:

  • selecting a fluid mixing ratio r;

    providing said buffer chamber with a first cross-sectional area A1;

    providing said mixing chamber with a second cross-sectional area A2;

    disposing said buffer in said buffer chamber;

    disposing said sample in said sample collection chamber;

    moving a buffer dispense plunger within said buffer chamber at a speed S1 towards said buffer port to dispense said buffer from said buffer chamber into said mixing chamber; and

    moving a sample dispense plunger substantially simultaneous with said buffer dispense plunger within said mixing chamber at a speed S2 away from said sample port to dispense said sample from said sample collection chamber into said mixing chamber, wherein said sample and buffer form a buffered sample solution;

    wherein A2S2=A1S1(1+1/r).

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