Compositions and methods for systemic administration of sequences encoding bone morphogenetic proteins

• US 20030170208A1
• Filed: 05/31/2002
• Published: 09/11/2003
• Est. Priority Date: 06/01/2001
• Status: Active Grant

• Current Assignee: Wyeth (Pfizer Inc.)
• Original Assignee: Wyeth (Pfizer Inc.)
• Inventors: Pittman, Debra D., Seeherman, Howard, Clancy, Brian
• Granted Patent: US 7,226,587 B2
• Time in Patent Office: Days
• US Class Current: 424/93.2
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2710/10343 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2800/108 episomal vectors

Abstract

Compositions and methods for systemic administration of DNA encoding bone morphogenic proteins for promotion of osteogenesis are disclosed. The compositions and methods of the invention may be utilized for fracture repair. The invention further discloses compositions and methods for systemic administration of bone morphogenetic proteins for promotion of osteogenesis. These compositions and methods may be used in bone fracture healing and repair. These composition of the invention may be further utilized in increasing bone mineral density.

Claims

1. A composition for promoting osteogenesis comprising a DNA sequence encoding a Bone Morphogenetic Protein (BMP).

5. The composition of claim 1 wherein said Bone Morphogenetic Protein is BMP-6.

6. The composition of claim 1 wherein said Bone Morphogenetic Protein is selected from the group consisting of BMP-2, BMP-4, BMP-5, BMP-7, BMP-10, BMP-12, BMP-13, BMP-15, and BMP-16.

12. A method for promoting osteogenesis in a patient in need of same, said method comprising systemic administration of the composition of claim 1.

20. The composition of claim 6 wherein said Bone Morphogenetic Protein is BMP-6.

21. The composition of claim 6 wherein said Bone Morphogenetic Protein is selected from the group consisting of BMP-2, BMP-4, BMP-5, BMP-7, BMP-10, BMP-12, BMP-13, BMP-15, and BMP-16.

2. A composition for fracture healing comprising a DNA sequence encoding a Bone Morphogenetic Protein.

7. The composition of claim 2 wherein said Bone Morphogenetic Protein is BMP-6.

8. The composition of claim 2 wherein said Bone Morphogenetic Protein is selected from the group consisting of BMP-2, BMP-4, BMP-5, BMP-7, BMP-10, BMP-12, BMP-13, BMP-15, and BMP-16.

13. A method for fracture healing in a patient in need of same, said method comprising systemic administration of the composition of claim 2.

22. The composition of claim 7 wherein said Bone Morphogenetic Protein is BMP-6.

23. The composition of claim 7 wherein said Bone Morphogenetic Protein is selected from the group consisting of BMP-2, BMP-4, BMP-5, BMP-7, BMP-10, BMP-12, BMP-13, BMP-15, and BMP-16.

3. A composition for increasing bone mineral density comprising a DNA sequence encoding a Bone Morphogenetic Protein.

14. A method for treating osteoporosis in a patient in need of same, said method comprising systemic administration of a therapeutically effective amount of the composition of claim 3.

4. A composition for treating osteoporosis comprising a DNA sequence encoding a Bone Morphogenetic Protein.

9. The composition of claim 4 wherein said BMP is BMP-6.

11. The composition of claim 9 wherein said DNA sequence is contained on an adenovirus vector.

10. A composition for promoting fracture repair healing comprising an injectable formulation comprising a DNA sequence encoding BMP-6 suitable for systemic administration.

15. A method for promoting fracture repair in a patient in need of same comprising a systemic administration of a DNA sequence encoding BMP-6.

16. A composition for promoting osteogenesis comprising a Bone Morphogenetic Protein suitable for systemic administration.

27. A method for promoting osteogenesis in a patient in need of same, said method comprising systemic administration of the composition of claim 16.

17. A composition for fracture healing comprising a Bone Morphogenetic Protein suitable for systemic administration.

18. A composition for increasing bone mineral density comprising a Bone Morphogenetic Protein suitable for systemic administration.

28. A method for fracture healing in a patient in need of same, said method comprising systemic administration of the composition of claim 18.

29. A method for treating osteoporosis in a patient in need of same, said method comprising systemic administration of id patient an effective amount of the composition of claim 18.

19. A composition for treating osteoporosis comprising a Bone Morphogenetic Protein suitable for systemic administration.

24. The composition of claim 19 wherein said BMP is BMP-6.

26. The composition of claim 19 wherein said protein is BMP-6.

25. A composition for promoting fracture repair healing comprising a BMP-6 protein BMP-6 suitable for systemic administration.

30. A method for promoting fracture repair in a protein t in need of same comprising systemic administration of BMP-6 in a suitable vector.