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In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development

  • US 20030170215A1
  • Filed: 12/20/2002
  • Published: 09/11/2003
  • Est. Priority Date: 12/21/2001
  • Status: Active Grant
First Claim
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1. A in situ method of providing insulin to a mammal comprising the steps of:

  • (a) encapsulating a cell culture of propagating pancreatic cells having the following properties;

    (i) the ability to be passed from one culture vessel to a second vessel at an initial concentration of about 180 cells per square centimeter and expanded to about 1,800 cells per square centimeter, and (ii) both the unexpanded and expanded cells are 90% PDX-1 positive and have an insulin;

    actin mRNA ratio of between 1;

    100 and 1000;

    1;

    (b) inserting the encapsulated cells into the mammal permitting the cells to mature to insulin secreting cells.

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