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Method of analyzing mRNA splice variants

  • US 20030170637A1
  • Filed: 03/06/2002
  • Published: 09/11/2003
  • Est. Priority Date: 03/06/2002
  • Status: Abandoned Application
First Claim
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1. A method for determining the exons present in a potentially variantly spliced mRNA, said method comprising the steps of:

  • (a) providing a potentially variantly spliced mRNA, said mRNA encoded by a DNA, said DNA comprising a plurality of exons, each of which plurality of exons may or may not be included in said mRNA;

    (b) providing an array, said array comprising a plurality of different primers immobilized on a solid support at distinct locations thereon, with each of said plurality of different primers selectively hybridizing to a corresponding one of said plurality of exons to form a duplex therebetween;

    (c) contacting said mRNA to said array so that a duplex is formed between each different primer and each corresponding exon if said corresponding exon is included in said mRNA;

    (d) subjecting said duplexes to a primer extension reaction so that the primers in said duplexes are extended with at least one labeled base; and

    then (e) detecting the presence or absence of said labeled base in each of said plurality of primers, the presence of said at least one labeled base indicating the presence of the exon to which said primer selectively binds in said potentially variantly spliced mRNA.

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