I. BF1H1 Cassette
First Claim
1. An isolated bovine VDJ gene cassette comprising the nucleic acid sequence shown in FIG. 1 (SEQ.ID.NO.1).
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Abstract
The present invention relates to a bovine VDJ cassette (BF1H1) that provides the novel ability to develop chimeric immunoglobulin molecule capable of incorporating both linear T cell epitope(s) (CDR1H and CDR2H) as well as conformational B cell epitope(s) (exceptionally long CDR3H). Further, multiple epitopes can be incorporated for development of multivalent vaccine by replacing at least a portion of an immunoglobulin molecule with the desired epitope such that functional ability of both epitope(s) and parent VDJ rearrangement is retained. The antigenized immunoglobulin incorporating both T and B epitopes of interest is especially useful for development of oral vaccines for use in humans apart from other species including cattle. The long CDR3H in BF1H1 VDJ rearrangement originates from long germline D-genes. The novel bovine germline D-genes provide additional opportunities for sustaining the capacity for antibody diversification in cattle essential for immunocompetence via selective breeding strategies that incorporate immunoglobulin gene markers. The novel gene elements, such as D-genes, are unique to cattle and, therefore, are useful in forensic analysis.
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Citations
13 Claims
- 1. An isolated bovine VDJ gene cassette comprising the nucleic acid sequence shown in FIG. 1 (SEQ.ID.NO.1).
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2. A recombinant plasmid pBF1H1-24 as shown in FIG. 1.
- 5. An isolated bovine germline D sequence comprising the nucleic acid sequence of D-1 (SEQ.ID.NO.2), D-2 (SEQ.ID.NO.3) and/or D-3 (SEQ.ID.NO.4) as shown in FIG. 2.
- 6. A recombinant plasmid pBGD14S as shown in FIG. 2.
- 7. A recombinant plasmid pBF1H1-D3 as shown in FIG. 3.
Specification