Conductivity-normalized urinary analyte concentration measurement for use in disease diagnosis

• US 20030171698A1
• Filed: 01/13/2003
• Published: 09/11/2003
• Est. Priority Date: 07/13/2000
• Status: Abandoned Application

• Current Assignee: Analyte Works Limited
• Original Assignee: Analyte Works Limited
• Inventors: Galili-Nachshon, Nitsa, Ben-Tzi Tzchori, Inbal, Nimri, Shai, Rubin, Yoram, Alon, Sari
• Application Number:
  US10/341,526
• Publication Number:
  US 20030171698A1
• Time in Patent Office: Days
• US Class Current: 600/584
• CPC Class Codes: G01N 33/88 involving prostaglandins or their receptors

Abstract

The invention relates to a method for the diagnosis of a medical condition in a subject, comprising the steps of measuring the concentration of one or more analytes, relevant for the diagnosis of said medical condition, in a urine sample; measuring the electrical conductivity of said urine sample; obtaining a normalized value for the analyte concentration by dividing said analyte concentration by the electrical conductivity; and determining whether said subject is suffering from said medical condition by means of comparing the normalized value with a pre-determined reference value. The medical condition to be diagnosed can be, for example, an acute cardiac condition for which the relevant urinary analyte can be one or more thromboxanes.

Claims

1. A method for the diagnosis of a medical condition in a subject, comprising the steps of:

a) obtaining a sample of urine from s. For one of the patients, the conductivity could not be determined.aid subject;

b) measuring the concentration of one or more analytes relevant for the diagnosis of said medical condition in said urine sample;

c) measuring the electrical conductivity of said urine sample;

d) obtaining a normalized value (NTV) for the analyte concentration by dividing said analyte concentration obtained in step b) by the electrical conductivity of the urine sample obtained in step c);

e) determining whether said subject is suffering from said medical condition by means of comparing the NTV obtained in step d) with a pre-determined reference value.

2. A method according to claim 1, wherein the medical condition to be diagnosed is an acute cardiac condition, and wherein the relevant urinary analyte comprises one or more thromboxanes selected from the group consisting of thromboxane B₂, 11-dehydrothromboxane B₂, 2,3-di-northromboxane B₂, and mixtures thereof,

3. A method according to claim 2, wherein the thromboxane measured is thromboxane B₂.

4. A method according to claim 2, wherein both the thromboxane concentration and the electrical conductivity are measured using an amperometric assay.

5. A method according to claim 2, wherein both the thromboxane concentration and the electrical conductivity are measured using a semiconductor-based device.

6. A method according to claim 2, wherein the electrical conductivity is measured using a conductivity meter.

7. A method according to claim 2, wherein the thromboxane concentration is determined using an immunoassay.

8. A method according to claim 7, wherein the immunoassay is an enzymeimmunoassay.