Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids

• US 20030172399A1
• Filed: 06/21/2002
• Published: 09/11/2003
• Est. Priority Date: 08/26/1999
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Fillatti, JoAnne J.
• Granted Patent: US 7,067,722 B2
• Time in Patent Office: Days
• US Class Current: 800/281
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)

Abstract

The present invention is directed to nucleic acid molecules and nucleic acid constructs, and other agents associated with fatty acid synthesis, particularly the ratios of saturated and unsaturated fats. Moreover, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of saturated and unsaturated fats. In particular, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of monounsaturated to polyunsaturated fatty acids.

Claims

1. A substantially purified nucleic acid molecule comprising a nucleic acid sequence with at least 70% sequence identity to SEQ ID NO:

4 or its complement.

2. The nucleic acid molecule of claim 1 wherein said sequence has at least 80% sequence identity to SEQ ID NO:

4 or its complement.

3. The nucleic acid molecule of claim 1 wherein said sequence has at least 90% sequence identity to SEQ ID NO:

4 or its complement.

4. The nucleic acid molecule of claim 1 wherein said sequence has at least 95% sequence identity to SEQ ID NO:

4 or its complement.

5. The nucleic acid molecule of claim 1 wherein said sequence has at least 99% sequence identity to SEQ ID NO:

4 or its complement.

6. A substantially purified nucleic acid molecule comprising a nucleic acid sequence with at least 70% sequence identity to SEQ ID NO:

12 or its complement.

7. The nucleic acid molecule of claim 6 wherein said sequence has at least 80% sequence identity to SEQ ID NO:

12 or its complement.

8. The nucleic acid molecule of claim 6 wherein said sequence has at least 90% sequence identity to SEQ ID NO:

12 or its complement.

9. The nucleic acid molecule of claim 6 wherein said sequence has at least 95% sequence identity to SEQ ID NO:

12 or its complement.

10. The nucleic acid molecule of claim 6 wherein said sequence has at least 99% sequence identity to SEQ ID NO:

12 or its complement.

11. A substantially purified nucleic acid molecule comprising a nucleic acid sequence with at least 70% sequence identity to SEQ ID NO:

13 or its complement.

12. The nucleic acid molecule of claim 11 wherein said sequence has at least 80% sequence identity to SEQ ID NO:

13 or its complement.

13. The nucleic acid molecule of claim 11 wherein said sequence has at least 90% sequence identity to SEQ ID NO:

13 or its complement.

14. The nucleic acid molecule of claim 11 wherein said sequence has at least 95% sequence identity to SEQ ID NO:

13 or its complement.

15. The nucleic acid molecule of claim 11 wherein said sequence has at least 99% sequence identity to SEQ ID NO:

13 or its complement.

16. A substantially purified nucleic acid molecule comprising a nucleic acid sequence with at least 70% sequence identity to SEQ ID NO:

14 or its complement.

17. The nucleic acid molecule of claim 16 wherein said sequence has at least 80% sequence identity to SEQ ID NO:

14 or its complement.

18. The nucleic acid molecule of claim 16 wherein said sequence has at least 90% sequence identity to SEQ ID NO:

14 or its complement.

19. The nucleic acid molecule of claim 16 wherein said sequence has at least 95% sequence identity to SEQ ID NO:

14 or its complement.

20. The nucleic acid molecule of claim 16 wherein said sequence has at least 99% sequence identity to SEQ ID NO:

14 or its complement.

21. A nucleic acid molecule comprising at least 15 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

4.

22. The nucleic acid molecule of claim 21, comprising at least 25 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

4.

23. The nucleic acid molecule of claim 21, comprising at least 50 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

4.

24. The nucleic acid molecule of claim 21, comprising at least 100 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

4.

25. A nucleic acid molecule comprising at least 15 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

12.

26. The nucleic acid molecule of claim 25, comprising at least 25 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

12.

27. The nucleic acid molecule of claim 25, comprising at least 50 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

12.

28. The nucleic acid molecule of claim 25, comprising at least 100 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

12.

29. A nucleic acid molecule comprising at least 15 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

13.

30. The nucleic acid molecule of claim 29, comprising at least 25 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

13.

31. The nucleic acid molecule of claim 29, comprising at least 50 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

13.

32. The nucleic acid molecule of claim 29, comprising at least 100 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

13.

33. A nucleic acid molecule comprising at least 15 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

14.

34. The nucleic acid molecule of claim 33, comprising at least 25 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

14.

35. The nucleic acid molecule of claim 33, comprising at least 50 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

14.

36. The nucleic acid molecule of claim 33, comprising at least 100 consecutive nucleotides of a nucleic acid molecule having the sequence of SEQ ID NO:

14.

37. A recombinant nucleic acid molecule comprising as operably linked components:

(A) a promoter that functions in a plant cell to cause production of an mRNA molecule; and

(B) a nucleic acid sequence that hybridizes under high stringency conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

4 and complements thereof.

38. A recombinant nucleic acid molecule comprising as operably linked components:

(A) a promoter that functions in a plant cell to cause production of an mRNA molecule; and

(B) a nucleic acid sequence that hybridizes under high stringency conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

12 and complements thereof.

39. A recombinant nucleic acid molecule comprising as operably linked components:

(A) a promoter that functions in a plant cell to cause production of an mRNA molecule; and

(B) a nucleic acid sequence that hybridizes under high stringency conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

13 and complements thereof.

40. A recombinant nucleic acid molecule comprising as operably linked components:

(A) a promoter that functions in a plant cell to cause production of an mRNA molecule; and

(B) a nucleic acid sequence that hybridizes under high stringency conditions to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

14 and complements thereof.

41. A transformed soybean plant having a nucleic acid molecule that comprises

(a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO:

1, 2, and complements and fragments thereof, and

(b) a second nucleic acid molecule with a second nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

4 through SEQ ID NO;

14 and complements and fragments thereof, wherein the second nucleic acid molecule is operably linked to the first promoter or a second promoter.

42. The transformed soybean plant according to claim 41, wherein a single promoter is operably linked to the first and second nucleic acid molecules.

43. The transformed soybean plant according to claim 42, wherein the single promoter is a seed specific promoter.

44. The transformed soybean plant according to claim 41, wherein the first promoter and the second promoter are both seed specific promoters.

45. The transformed soybean plant according to claim 41, wherein the first promoter and the second promoter are the same.

46. The transformed soybean plant according to claim 45, wherein the first promoter and the second promoter are both 7S promoters.

47. The transformed soybean plant according to claim 41, wherein the first promoter is different from the second promoter.

48. The transformed soybean plant according to claim 47, wherein the first promoter is a 7S promoter and the second promoter is a napin promoter.

49. The transformed soybean plant according to claim 41, wherein said first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a FAD2-1 gene while leaving the level of a transcript encoded by a FAD2-2 gene partially unaffected.

50. The transformed soybean plant according to claim 41, wherein said first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a FAD2-1 gene while leaving the level of a transcript encoded by a FAD2-2 gene substantially unaffected.

51. The transformed soybean plant according to claim 41, wherein said first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a FAD2-1 gene while leaving the level of a transcript encoded by a FAD2-2 gene essentially unaffected.

52. A transformed soybean plant having two or more nucleic acid molecules wherein each nucleic acid molecule is operably linked to a promoter and wherein each nucleic acid molecule has a nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO:

1, 2, and 4-14 and complements and fragments thereof.

53. The transformed soybean plant according to claim 52, wherein a first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a first FAD gene while leaving the level of a transcript encoded by a second FAD gene partially unaffected.

54. The transformed soybean plant according to claim 52, wherein a first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a first FAD gene while leaving the level of a transcript encoded by a second FAD gene substantially unaffected.

55. The transformed soybean plant according to claim 52, wherein a first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a first FAD gene while leaving the level of a transcript encoded by a second FAD gene essentially unaffected.

56. A transformed soybean plant, wherein the level of a transcript encoded by a gene selected from the group consisting of FAD2-1A, FAD2-1B, FAD2-2B, FAD3-1A, FAD3-1B, FAD3-1C is selectively reduced while leaving the level of a transcript encoded by a different gene selected from the group consisting of FAD2-1A, FAD2-1B, FAD2-2B, FAD3-1A, FAD3-1B, FAD3-1C at least partially unaffected.

57. A method of producing a soybean plant having a seed with reduced linolenic acid content comprising:

transforming a soybean plant with a nucleic acid molecule that comprises

(a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

1, 2 and complements thereof, and

(b) a second nucleic acid molecule having a second nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

4 through SEQ ID NO;

14 and complements thereof, wherein the second nucleic acid molecule is operably linked to the first promoter or a second promoter; and

growing said plant, wherein said plant produces seed with less linolenic acid than a plant having a similar genetic background but lacking said nucleic acid molecule.

58. A method of producing a soybean plant having a seed with increased oleic acid content comprising:

transforming a soybean plant with a nucleic acid molecule that comprises

(a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

1, 2 and complements thereof, and

(b) a second nucleic acid molecule having a second nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

4 through SEQ ID NO;

14 and complements thereof, wherein the second nucleic acid molecule is operably linked to the first promoter or a second promoter; and

growing said plant, wherein said plant produces seed with more oleic acid than a plant having a similar genetic background but lacking said nucleic acid molecule.

59. A method of producing a plant having a seed with a modified oil composition comprising:

transforming a plant with a nucleic acid molecule that comprises, as operably linked components, a first promoter and a first nucleic acid molecule having a first nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

1, 2, 4 through 14 and complements thereof; and

, growing said plant, wherein said plant produces seed with a modified oil composition compared to a plant having a similar genetic background but lacking said nucleic acid molecule.

60. A method of producing a plant having a seed with an altered ratio of monounsaturated to polyunsaturated fatty acids comprising:

transforming a plant with a construct that comprises, as operably linked components, two or more nucleic acid molecules, each having a nucleic acid sequence that has 85% or greater identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO;

1, 2, 4 through 14 and complements thereof, wherein each nucleic acid molecule is operably linked to a promoter; and

, growing said plant, wherein said plant produces seed with an altered ratio of monounsaturated to polyunsaturated fatty acids compared to a plant having a similar genetic background but lacking said two or more nucleic acid molecules.