Printing molecular library arrays
First Claim
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1. A method of forming polymers having diverse monomer sequences on a substrate, said method comprising:
- providing a substrate comprising a linker molecule layer thereon, said linker molecule layer comprising a linker molecule and a protective group;
applying a barrier layer overlying said linker molecule layer, said applying step forming selected exposed regions of said linker molecule layer; and
exposing said selected exposed regions of said linker molecule layer to a vapor comprising a deprotecting agent.
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Abstract
A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.
32 Citations
28 Claims
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1. A method of forming polymers having diverse monomer sequences on a substrate, said method comprising:
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providing a substrate comprising a linker molecule layer thereon, said linker molecule layer comprising a linker molecule and a protective group;
applying a barrier layer overlying said linker molecule layer, said applying step forming selected exposed regions of said linker molecule layer; and
exposing said selected exposed regions of said linker molecule layer to a vapor comprising a deprotecting agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of deprotecting selected regions of a substrate, said method comprising:
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providing a substrate comprising a layer of linker molecules thereon, each of said linker molecules having a protective group;
applying a vapor deprotection agent to selected regions of said linker molecule layer. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A method of applying a medium in selected regions of a substrate, said method comprising the steps of:
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providing a partially completed substrate comprising an array of monomers, said partially completed substrate having a top surface;
selectively applying a medium comprising an element selected from a group consisting of a barrier material, a receptor, a deprotection agent, a monomer group, a carrier material, and an activator to selected regions of said substrate top surface. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. A method of synthesizing an oligonucleotide comprising the steps of:
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coupling a first portion of said oligonucleotide to said substrate, said first portion of said oligonucleotide comprising a removable protecting group;
removing said protecting group with a vapor phase deprotection agent to expose a functional group on said first portion of said oligonucleotide; and
covalently bonding a second portion of said oligonucleotide to said first portion of said oligonucleotide. - View Dependent Claims (24, 25, 26, 27, 28)
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Specification