Quantifying target molecules contained in a liquid

• US 20030175737A1
• Filed: 08/30/2002
• Published: 09/18/2003
• Est. Priority Date: 03/01/2000
• Status: Abandoned Application

• Current Assignee: November Aktiengesellschaft Gesellschaft Fur Molekulare Medizin
• Original Assignee: November Aktiengesellschaft Gesellschaft Fur Molekulare Medizin
• Inventors: Hassmann, Jorg, Schulein, Jurgen, Grassl, Bjorn
• Application Number:
  US10/220,401
• Publication Number:
  US 20030175737A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The invention relates to a method for detecting and/or quantifying first biopolymers contained in a liquid involving the following steps: a) providing an electrode with a surface which is made of plastic and which is coated with second biopolymers that have a specific affinity to the first biopoymers to be detected; b) bringing the electrode into contact with the liquid; c) applying a predetermined voltage protocol to the electrode in order to effect a concentration of the first biopolymers on the second biopolymers; d) adding osmium tetroxide and bipyridine to the liquid, and; e) measuring the redox signal coming off the electrode.

Claims

1. A process for the detection and/or quantification of first biopolymers present in a liquid, having the following steps:

a) provision of an electrode having a surface made of plastic which is coated with second biopolymers which have a specific affinity to the first biopolymers to be detected,

b) bringing of the electrode into contact with the liquid,

c) application of a pre-specified voltage program to the electrode, causing enrichment of the first biopolymers at the second biopolymers,

d) addition of osmium tetroxide and bipyridine to the liquid,

e) measurement of the redox signal falling off at the electrode.

2. A process as claimed in claim 1, in which the plastic is an electrically conductive composite material.

3. A process as claimed in one of the preceding claims, in which the electrode consists entirely of plastic.

4. A process as claimed in one of the preceding claims, in which the second biopolymers are bonded to a matrix, preferably made of dextran or polyethylene glycol, applied to the surface of the electrode.

5. A process as claimed in one of the preceding claims, in which one of the following measurements is carried out in step e:

direct-voltage measurement, cyclovoltammetric measurement, chronoamperometric measurement, chronovoltammetric measurement.

6. A process as claimed one of claims 1 to 3, in which a differential pulse voltammogram is recorded in step e.

7. A process as claimed in one of claims 1 to 3, in which an impendance spectrum is recorded in step e.

8. A process as claimed in one of claims 1 to 3, in which an alternating-current signal is measured phase-sensitively in step e.

9. A process as claimed in claim 5, in which the alternating-current signal is superimposed on a direct-voltage signal.

10. A process as claimed in one of the preceding claims, in which, in order to quantify the first biopolymers, integration is carried out via a peak of a measurement signal.

11. A process as claimed in one of the preceding claims, in which, for multiple measurement, the electrode is rinsed or heated after step e.

12. A process as claimed in one of the preceding claims, in which the first biopolymers are subjected to a polymerase chain reaction before step a.