Method of detection of nucleic acids with a specific sequence composition

• US 20030175789A1
• Filed: 04/03/2003
• Published: 09/18/2003
• Est. Priority Date: 12/09/1994
• Status: Abandoned Application

• Current Assignee: Arthur M. Weininger, Susan Weininger
• Original Assignee: Arthur M. Weininger, Susan Weininger
• Inventors: Weininger, Arthur M., Weininger, Susan
• Application Number:
  US10/407,543
• Publication Number:
  US 20030175789A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: C12Q 1/6804 Nucleic acid analysis using immunogens immunoassay G01N33/53
• CPC Class Codes: C12Q 1/6811 Selection methods for production or design of target specific oligonucleotides or binding molecules
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6853 using modified primers or templates
• CPC Class Codes: C12Q 1/703 Viruses associated with AIDS
• CPC Class Codes: C12Q 1/708 for papilloma
• CPC Class Codes: C12Q 2525/301 Hairpin oligonucleotides
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2537/149 Sequential reactions
• CPC Class Codes: C12Q 2537/161 A competitive reaction step

Abstract

This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.

Claims

1. A method of using a target binding assembly (TBA) wherein said TBA comprises a plurality of nucleic acid recognition units assembled by assembly sequences that are not ligated together, wherein each of said nucleic acid recognition units binds to a specific nucleic acid sequence on a target double stranded nucleic acid molecule;

and wherein the combined affinity of said plurality of nucleic acid recognition units is such that said TBA specifically binds to the target double stranded nucleic acid molecule but does not bind to non-target molecules; and

wherein said method comprises administering to a patient a therapeutically or prophylactically effective amount of said TBA, or nucleic acid which codes for and produces said TBA, such that the TBA binds a target double stranded nucleic acid molecule to achieve a desired prophylactic or therapeutic result.