Devices and methods for isolating target cells
First Claim
1. A device for isolating and identifying a target cell from a cellular mixture, said device comprising:
- a substantially planar surface having a bioactive coating applied thereon; and
at least one bifunctional compound capable of binding to said target cell and to said bioactive coating; and
a delivery conduit communicating said bifunctional compound with said substantially planar surface.
2 Assignments
0 Petitions
Accused Products
Abstract
A cell isolating device and method is provided to concentrate or isolate cells with specific characteristics from a mixture of different cell types. One embodiment may comprise two subtypes of antibodies that are directly conjugated to biotin (Abb) and conjugated to a fluorescent molecule (Abf). The conjugated antibodies (Abb+Abf) bind to the target cells in a mixed cell suspension. The cell suspension is then passed over an immobilized avidin or streptavidin substrate on a glass microscope slide. The biotinylated target cells adhere to the avidin/streptavidin substrate, while the unbound cells are washed off and collected in a wicking member. Captured cells on the avidin/streptavidin substrate may then be visualized directly using a fluorescent microscope or detected and enumerated via an on-board fluorescent detection device.
67 Citations
29 Claims
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1. A device for isolating and identifying a target cell from a cellular mixture, said device comprising:
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a substantially planar surface having a bioactive coating applied thereon; and
at least one bifunctional compound capable of binding to said target cell and to said bioactive coating; and
a delivery conduit communicating said bifunctional compound with said substantially planar surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A device for isolating and identifying a target cell from a cellular mixture, said device comprising:
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a substantially planar surface having a bioactive coating applied thereon;
a conduit in spaced relationship with said surface, wherein said conduit includes at least one channel capable of delivering said cellular mixture to said bioactive coating; and
a fluid absorbing media provided on said surface and positioned adjacent to said bioactive coating. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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20. A method of isolating a target cell from a cellular mixture, said method comprising:
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providing a substantially planar surface having a bioactive coating applied thereon;
providing a plurality of bifunctional compounds capable of binding to said target cell and to said bioactive coating;
combining said cellular mixture with said bifunctional compound;
exposing said cellular mixture to said bioactive coating through a delivery conduit; and
analyzing said bioactive coating for said target cell. - View Dependent Claims (21, 22, 23, 24, 25, 26)
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27. A method of isolating target cells from a cellular mixture comprising:
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exposing a cellular mixture to a targeting solution to form a target cell mixture;
delivering said target cell mixture to a substantially planar reaction surface;
controlling the delivery of said target mixture so as to optimize the flow over said reaction surface;
retaining said target cells on said reaction surface; and
analyzing said target cells remaining on said reaction surface. - View Dependent Claims (28, 29)
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Specification