Delayed release of fluids

• US 20030175853A1
• Filed: 11/19/2002
• Published: 09/18/2003
• Est. Priority Date: 01/25/2000
• Status: Abandoned Application

• Current Assignee: The Victoria University of Manchester
• Original Assignee: The Victoria University of Manchester
• Inventors: Smith, Andrew, Clarke, David J
• Application Number:
  US10/182,187
• Publication Number:
  US 20030175853A1
• Time in Patent Office: Days
• US Class Current: 435/41
• CPC Class Codes: C12M 23/34 Internal compartments or partitions

Abstract

A method of effecting delayed release of a fluid (19) comprises providing the fluid in a vessel (12) having a discharge aperture (14) provided with a polymeric barrier (5) to fluid release. The barrier (15) is caused to degrade to provide for failure of the barrier and discharge of the fluid. The liquid to be discharged may be an aqueous medium and the barrier (15) may be of a polymeric material capable of forming in the presence of the aqueous medium a gel that will dissolve therein so as to cause failure of the barrier. The polymeric material may be a cellulose ether. The fluid may, for example, comprise a culture of microorganisms which are subjected to pre-enrichment in the vessel for a predetermined period of time prior to failure of the barrier (15) and discharge of the culture into, for example, a selective medium.

Claims

1. A method of treating a culture of microbial organisms in a liquid medium comprising providing the liquid medium (containing the microbial organisms) in a vessel having a discharge aperture provided with a polymeric barrier to liquid flow, effecting the treatment, and utilising liquid in the vessel to cause the barrier to degrade to undergo a loss of mechanical strength or integrity to provide for failure of the barrier and discharge of the liquid medium.

2. A method as claimed in claim 1 wherein the degradation of the barrier is caused by at least partial dissolution thereof in the liquid.

3. A method as claimed in claim 1 wherein the liquid converts the barrier to a gel and the gel at least partially dissolves in the liquid to provide for failure of the barrier.

4. A method as claimed in claim 3 wherein the liquid is an aqueous medium.

5. A method as claimed in claim 4 wherein the polymeric barrier is capable of forming, in the presence of the aqueous medium, a hydrated gel which will dissolve in the medium to provide for failure of the barrier.

6. A method as claimed in claim 5 wherein the polymeric material is a cellulose ether containing methoxyl groups.

7. A method as claimed in claim 6 wherein the cellulose ether contains 15% to 35% substitution by methoxyl groups.

8. A method as claimed in claim 6 or 7, wherein the cellulose ether further contains hydroxypropyloxyl groups.

9. A method as claimed in claim 8, wherein the cellulose ether contains 4% to 15% substitution by hydroxypropyloxy groups.

10. A method as claimed in claim 9 wherein the cellulose ether contains 15% to 35% substitution by methoxyl groups and 4% to 15% substitution by hydroxypropyloxy groups.

11. A method as claimed in claim 10, wherein the cellulose ether contains 19% to 24% methoxyl substitution and 7% to 12% hydroxypropyloxy substitution.

12. A method as claimed in any one of claims 6 to 11, wherein the cellulose ether has a viscosity of 50 to 100,000 cp for a 2% solution.

13. A method as claimed in any one of claims 1 to 12 wherein the treatment effected to the culture is recovery, resuscitation, growth or enrichment.

14. A method as claimed in claim 13 wherein on failure of the barrier the culture is discharged into a selective medium.

15. A method as claimed in claim 13 or 14 wherein provision is made for sustained release of an agent into the culture.

16. A method as claimed in claim 15 wherein the agent for sustained release is provided in a tablet or the like provided in said medium.

17. A method as claimed in claim 15 wherein the agent for sustained release is provided in the barrier layer.

18. A method as claimed in any one of claims 15 to 17 wherein the agent for sustained release is a carbon source to encourage growth.

19. A method as claimed in any one of claim 15 to 17 wherein the agent for sustained release is a selective agent.

20. A method as claimed in any one of claims 1 to 19 wherein, on failure of the barrier, the culture is discharged through the aperture under gravity.

21. A method as claimed in any one of claims 1 to 20 wherein the barrier layer is provided with a layer of a pH responsive polymer.

22. A method as claimed in claim 1 wherein the barrier is of an at least partially crystalline material and the fluid causing degradation of the barrier disrupts the crystallinity to provide for barrier failure.

23. A method as claimed in any one of claims 1 to 22 wherein degradation of the barrier is enhanced by energy provided from externally of the vessel.

24. A method as claimed in claim 23 wherein said energy in a laser beam.

25. A method as claimed in claim 23 wherein said energy is ultrasound.

27. A method as claimed in any one of claims 1 to 26 wherein, at the outset, the polymeric barrier is a tight fit in the discharge aperture.

28. A method as claimed in any one of claims 1 to 27 wherein the polymeric barrier is provided in the form of a replaceable cartridge.

29. Apparatus for use in the method of any one of claims 1 to 28 comprised of a vessel capable of holding a liquid medium and having a discharge aperture provided with a polymeric barrier to liquid release, said barrier being capable of undergoing degradation by the liquid in the vessel to provide for failure of the barrier and discharge of liquid medium wherein the vessel provides a first chamber, the apparatus further comprises a second chamber connected to the first chamber via said discharge aperture in which the polymeric barrier is provided, and the walls of the first and second chambers are of a flexible material.

30. Apparatus as claimed in claim 29, wherein the second chamber is external of the first chamber.

31. Apparatus as claimed in claim 29 to 30, wherein the first chamber is an upper chamber and the second chamber is a lower chamber.

32. Apparatus as claimed in claim 31 wherein the second chamber is sub-divided into a plurality of compartments.

33. Apparatus as claimed in claim 32 wherein the first, upper chamber is subdivided into a plurality of compartments and each such compartment has a respective discharge aperture provided with a barrier of the polymeric material and being positioned one above each of the compartments of the second chamber.

34. Apparatus for use in the method of any one of claims 1 to 28 comprised of a vessel capable of holding a liquid medium and having a discharge aperture provided with a polymeric barrier to liquid release, said barrier being capable of undergoing degradation by the liquid in the vessel to provide for failure of the barrier and discharge of liquid medium wherein the vessel provides a first upper chamber, the apparatus comprises a second lower chamber sub-divided into a plurality of compartments and the first, upper chamber is sub-divided into a plurality of compartments, each such compartment of the first chamber having a respective discharge aperture provided with a barrier of the polymeric material and being positioned one above each of the compartments of the second chamber.

35. Apparatus as claimed in claim 34, wherein the walls of the first and second chambers are of a flexible material.

36. Apparatus as claimed in any one of claims 29 to 35 wherein the polymeric barrier is a tight fit in the discharge aperture.

26. A method of isolating microbial organisms involving the steps of (i) resuscitation or pre-enrichment and (ii) selective enrichment, wherein the step (i) is effected using an aqueous media in a vessel having a liquid discharge aperture provided with a barrier to liquid flow of a polymeric material capable of forming, in the presence of the aqueous medium, a hydrated gel which will dissolve in the medium to provide for failure of the barrier, and on failure of the plug discharging the culture from step (i) into a selective medium.

37. Apparatus as claimed in any one of clam 29 to 36 wherein the polymeric barrier is provided in the form of a replaceable cartridge.

38. A cartridge for use in the apparatus of claim 37, said cartridge comprising the polymeric barrier material and a frame encircling said material.