Recycling system for manipulation of intracellular NADH availability
First Claim
1. A method of increasing the availability of NADH in a cell, comprising the transformation of a cell with a nucleotide sequence encoding an NAD+-dependent dehydrogenase and growth of said cell under conditions in which said NAD+-dependent dehydrogenase increases the intracellular availability of NADH.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a vitamin, a pharmaceutical and a biodegraded organic molecule. The NADH recycling system effects metabolic flux of reductive pathways in aerobic and anaerobic environments.
-
Citations
47 Claims
- 1. A method of increasing the availability of NADH in a cell, comprising the transformation of a cell with a nucleotide sequence encoding an NAD+-dependent dehydrogenase and growth of said cell under conditions in which said NAD+-dependent dehydrogenase increases the intracellular availability of NADH.
- 11. A cell comprising a recombinant NADH-recycling system, wherein said system comprises a heterologous nucleotide sequence encoding an NAD+-dependent dehydrogenase.
- 20. A method for producing a compound comprising growing a culture of cells in a culture medium, wherein the culture comprises at least one cell comprising a recombinant NADH recycling system, and obtaining said compound from said culture.
- 29. A method for biodegrading a compound comprising growing a culture of cells in a culture medium, wherein the culture comprises at least one cell comprising a recombinant NADH recycling system, and obtaining said biodegraded compound from said culture.
- 38. A method for altering metabolic flux in a cell comprising growing a culture of cells in a culture medium, wherein the culture comprises at least one cell comprising a recombinant NADH recycling system, wherein the increased intracellular availability of NADH results in a redistribution of metabolism towards a more anaerobic state.
Specification