Feeder-free culture method for embryonic stem cell
First Claim
1. A method of causing primate primordial stem (pPS) cells to proliferate in an undifferentiated form, comprising culturing them on an extracellular matrix with a nutrient medium in a growth environment free of feeder cells.
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Abstract
Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt.
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Citations
20 Claims
- 1. A method of causing primate primordial stem (pPS) cells to proliferate in an undifferentiated form, comprising culturing them on an extracellular matrix with a nutrient medium in a growth environment free of feeder cells.
Specification