Platform for the differentiation of cells

• US 20030175963A1
• Filed: 03/11/2003
• Published: 09/18/2003
• Est. Priority Date: 02/04/1999
• Status: Abandoned Application

• Current Assignee: McGill University
• Original Assignee: McGill University
• Inventors: Rosenberg, Lawrence
• Application Number:
  US10/386,759
• Publication Number:
  US 20030175963A1
• Time in Patent Office: Days
• US Class Current: 435/375
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 5/48 of the pancreatic hormones
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/13 Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
• CPC Class Codes: C12N 2501/33 Insulin
• CPC Class Codes: C12N 2501/58 Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
• CPC Class Codes: C12N 2506/22 from pancreatic cells
• CPC Class Codes: C12N 5/0676 Pancreatic cells

Abstract

The present invention relates to an in vitro method for islet cell expansion, which comprises the steps of: a) preparing dedifferentiated cells derived from cells in or associated with post-natal islets of Langerhans; b) expanding the dedifferentiated cells; and c) inducing islet cell differentiation the expanded cells of step b) to become insulin-producing cells.

Claims

1. An in vitro method for producing cells with at least bipotentiality, which comprises the step of:

a) inducing cystic formation in cells in or associated with post-natal islets of Langerhans obtained from a patient to obtain a duct-like structure.

2. A method for the treatment of diabetes mellitus in a patient, which comprises the steps of:

a) inducing cystic formation in post-natal islets of Langerhans cells obtained from said patient to obtain a duct-like structure; and

b) introducing said duct-like structure cells in situ in said patient, wherein said cells are expanded in situ to become insulin-producing cells.

3. A method for the treatment of diabetes mellitus in a patient, which comprises the steps of:

a) inducing in vitro cystic formation in post-natal islets of Langerhans cells obtained from said patient to become insulin-producing cells; and

b) introducing said cells of step a) in situ in saidpatient, wherein said cells produce insulin in situ.