Enzyme electrode
First Claim
1. An enzyme electrode comprising:
- (a) an electrode system comprising an insulating substrate having formed thereon a working electrode, a counter electrode and optionally a reference electrode; and
(b) a reaction layer formed on said electrode system, wherein said reaction layer comprises diaphorase (DI), 12α
-hydroxysteroid dehydrogenase (12α
-HSD) and nicotinamide adenine dinucleotide synthetase (NADS), at least a portion of said reaction layer being superposed on said working electrode, wherein the DI, 12α
-HSD and NADS contained in said portion of the reaction layer are immobilized on the surface of said working electrode, so that a compound generated in said reaction layer can reach the surface of said working electrode.
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Accused Products
Abstract
Disclosed is an enzyme electrode comprising an electrode system comprising an insulating substrate having formed thereon a working electrode, a counter electrode and optionally a reference electrode, and a reaction layer formed on the electrode system, wherein the reaction layer comprises diaphorase (DI), 12α-hydroxysteroid dehydrogenase (12α-HSD) and nicotinamide adenine dinucleotide synthetase (NADS), at least a portion of the reaction layer being superposed on the working electrode, wherein the DI, 12α-HSD and NADS contained in the reaction layer are immobilized on the surface of the working electrode, so that a compound generated in the reaction layer can reach the surface of the working electrode. Also disclosed are a method for and a system for determining the concentration of adenosine triphosphate (ATP) in a sample by using the above-mentioned enzyme electrode.
7 Citations
8 Claims
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1. An enzyme electrode comprising:
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(a) an electrode system comprising an insulating substrate having formed thereon a working electrode, a counter electrode and optionally a reference electrode; and
(b) a reaction layer formed on said electrode system, wherein said reaction layer comprises diaphorase (DI), 12α
-hydroxysteroid dehydrogenase (12α
-HSD) and nicotinamide adenine dinucleotide synthetase (NADS),at least a portion of said reaction layer being superposed on said working electrode, wherein the DI, 12α
-HSD and NADS contained in said portion of the reaction layer are immobilized on the surface of said working electrode, so that a compound generated in said reaction layer can reach the surface of said working electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification