Method for measuring substance and testing piece

• US 20030180183A1
• Filed: 03/11/2003
• Published: 09/25/2003
• Est. Priority Date: 10/30/1995
• Status: Abandoned Application

• Current Assignee: Atsuko Katayama, Kenji Yamamoto, Satoshi Yonehara, Takao Fukuoka
• Original Assignee: Atsuko Katayama, Kenji Yamamoto, Satoshi Yonehara, Takao Fukuoka
• Inventors: Yamamoto, Kenji, Yonehara, Satoshi, Fukuoka, Takao, Katayama, Atsuko
• Application Number:
  US10/384,606
• Publication Number:
  US 20030180183A1
• Time in Patent Office: Days
• US Class Current: 422/56
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple analysis is made possible, and easy handling is possible.

Claims

1. An analytical testing piece for measuring an analyte by measuring a detectable substance formed by a reaction of the analyte contained in a sample with a reagent, wherein the testing piece comprises at least one test portion having a detection area for detecting the detectable substance and a reaction portion where the analyte contained in the sample reacts with the reagent, and the detectable substance is formed in the reaction portion, and the testing piece contains a layered inorganic compound at least in the test portion.

2. The testing piece according to claim 1, wherein the test portion comprises a time control portion for adjusting the proceeding of a reaction making use of a time during which the sample moves so that the detectable substance passes through the time control portion and reaches the detection area.

3. The testing piece according to claim 1, wherein the test portion has a diffusion area for diffusing the sample so that the sample passes through the diffusion area to be diffused and reaches the detection area.

4. The testing piece according to claim 1, wherein the detection area is composed of two or more layers including a detection layer for detecting the detectable substance.

5. The testing piece according to claim 1, wherein the detection area is provided at a location which the sample reaches after the sample is diffused and passes through the reaction portion.

6. The testing piece according to claim 1, wherein a portion containing the layered inorganic compound in the testing piece is composed of a porous structure.

7. The testing piece according to claim 6, wherein the porous structure is formed by the layered inorganic compound or at least one member selected from the group consisting of a hydrophilic polymer, a membrane filter, a fiber assembly and organic or inorganic compound fine powders.

8. The testing piece according to claim 1, wherein the layered inorganic compound is a 2:

1 type clay mineral.

9. The testing piece according to claim 8, wherein the 2:

1 type clay mineral is a swelling layered clay mineral.

10. The testing piece according to claim 9, wherein the swelling layered clay mineral is at least one member selected from the group consisting of bentonite, smectite, vermicuilite and synthetic fluorine mica.

11. The testing piece according to claim 10, wherein the smectite is synthetic smectite.

12. The testing piece according to claim 11, wherein the synthetic smectite is at least one member selected from the group consisting of hectorite and saponite.

13. The testing piece according to claim 1, wherein the reagent is contained in the test portion by adding a solution of the reagent before and/or after the addition of the sample to the test portion.

14. The testing piece according to claim 1, wherein a buffer or a dried product thereof is further contained in a portion containing the layered inorganic compound.

15. The testing piece according to claim 1, wherein the reagent is capable of forming a substance detectable by an optical method when the reagent reacts with the analyte.

16. The testing piece according to claim 15, wherein the substance detectable by the optical method is water-soluble.

17. An analytical testing piece for measuring an analyte by measuring a detectable substance formed by a reaction of the analyte contained in a sample with a reagent, wherein the testing piece comprises at least one test portion having a detection area for detecting the detectable substance and a diffusion area for diffusing the sample so that the sample passes through the diffusion area to be diffused and reaches the detection area, and the testing piece contains a layered inorganic compound at least in the test portion.