Chemical sensor device

• US 20030180184A1
• Filed: 11/08/2002
• Published: 09/25/2003
• Est. Priority Date: 05/11/2000
• Status: Active Grant

• Current Assignee: Panasonic Ecology Systems Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Ecology Systems Company Limited (Panasonic Holdings Corporation)
• Inventors: Nishiguchi, Masashi, Onouchi, Toru, Niwa, Kazuhiro
• Granted Patent: US 7,662,612 B2
• Time in Patent Office: Days
• US Class Current: 422/68.1
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A sensor device is provided for performing rapid and high-sensitivity detection. The sensor device comprising at least one support for immobilizing a subject to be detected and a cell for containing a solution in which a reaction product generated from the subject to be detected is diffused. At least one reaction region having a constant concentration of the reaction product is formed by diffusion of the reaction product into the solution, and the reaction region is formed in such a manner that the reaction product is specifically detected in a predetermined measurement time.

Claims

1. A sensor device, comprising:

at least one support for fixing a subject to be detected; and

a cell for containing a solution in which a reaction product generated from the subject to be detected is diffused,

wherein at least one reaction region having a constant concentration of the reaction product is formed by diffusion of the reaction product into the solution, and the reaction region is formed in such a manner that the reaction product is specifically detected in a predetermined measurement time.

2. A sensor device according to claim 1, wherein a plurality of reaction regions are formed.

3. A sensor device according to claim 2, wherein the plurality of reaction regions are separately formed by different reaction products.

4. A sensor device according to claim 1, further comprising detection means.

5. A sensor device according to claim 1, wherein the subject to be detected is immobilized by fixing means.

6. A sensor device according to claim 4, wherein the detection means has a detection region, and the detection region includes the reaction region.

7. A sensor device according to claim 4, wherein the detection means has a detection region, and the detection region overlaps the reaction region.

8. A sensor device according to claim 4, wherein the detection means has a detection region, and the detection region is included in the reaction region.

9. A sensor device according to claim 1, wherein the support is a particular region on a base plate.

10. A sensor device according to claim 1, wherein the support is a particulate.

11. A sensor device according to claim 1, wherein the support is a rod-shaped member.

12. A sensor device according to claim 1, wherein the measurement time is 30 minutes.

13. A sensor device according to claim 1, wherein the measurement time is 10 minutes.

14. A sensor device according to claim 1, wherein the measurement time is 5 minutes.

15. A sensor device according to claim 1, wherein the measurement time is 3 minutes.

16. A sensor device according to claim 1, wherein the measurement time is 1 minute.

17. A sensor device according to claim 4, wherein the detection means measures light.

18. A sensor device according to claim 4, wherein the detection means measures heat.

19. A sensor device according to claim 4, wherein the detection means comprises at least one electrode.

20. A sensor device according to claim 19, wherein the electrode acts on the reaction product and generates an electrical signal corresponding to the amount of the reaction product.

21. A sensor device according to claim 1, wherein the subject to be detected is an enzyme, and the reaction product is an enzyme reaction product.

22. A sensor device according to claim 1, wherein the subject to be detected is an antibody or an enzyme linked to a peptide.

23. A sensor device according to claim 22, wherein the fixing means is an antigen.

24. A sensor device according to claim 22, wherein the fixing means is an antigen-antibody complex.

25. A sensor device according to claim 1, wherein the subject to be detected is an enzyme linked to a first antibody linked to an antigen, and the fixing means is a second antibody.

26. A sensor device according to claim 4, wherein the subject to be detected is immobilized in a region having a diameter of several tens to several hundreds of μ

m, and the detection means is an electrode having a diameter of 1 mm or less.

27. A sensor device according to claim 26, wherein when the subject to be detected is measured, the electrode approaches the region in which the subject to be detected is immobilized.

28. A sensor device according to claim 1, wherein the electrode is provided on the support, and the subject to be detected is immobilized in a region having a diameter of several tens to several hundreds of μ

m.

29. A sensor device according to claim 4, wherein the subject to be detected is immobilized in a region having a diameter of several tens to several hundreds of μ

m, and the electrode surrounds the region.

30. A sensor device according to claim 1, wherein the support is a material selected from the group consisting of glass, ceramics, noble metals, and resins.

31. A sensor device according to claim 1, further comprising means for promoting the fixing of the subject to be detected.

32. A sensor device according to claim 30, wherein the promoting means is used to stir the solution in the cell.

33. A sensor device according to claim 31, wherein the promoting means is used to exchange the solution in the cell.

34. A sensor device according to claim 31, wherein the promoting means is used to supply the solution in the cell.

35. A sensor device according to claim 31, wherein the promoting means is used to allow the solution to flow through the cell.