Analyte-shaped cavities associated with enhancing particle structures for analyte detection
First Claim
1. A device for analyte detection, comprising:
- an analyte-shaped cavity; and
an enhancing structure associated with said analyte-shaped cavity.
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Accused Products
Abstract
This invention comprises novel matrices comprising enhancing structures associated with analyte-shaped cavities. The structures are useful for Raman spectroscopic analyses of analytes in complex solutions. Analytes that can be detected using these methods include nucleic acids, proteins, and other molecules or viruses that can specifically bind to the arrays. Enhancing structures are disclosed that enhance the Raman signal produced by an analyte through surface and resonance phenomena. Analyte-shaped cavities associated with enhancing structures can be formed, which are complementary to a large number of desired analytes. Novel methods are presented for manufacturing matrices and biochips comprising enhancing structures associated with analyte-shaped cavities. The matrices and biochips of this invention can produce Raman signals that can be used for highly specific, sensitive assays of biological molecules and viruses.
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Citations
23 Claims
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1. A device for analyte detection, comprising:
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an analyte-shaped cavity; and
an enhancing structure associated with said analyte-shaped cavity. - View Dependent Claims (2, 3, 4, 5)
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6. A biochip comprising:
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a substrate an analyte-shaped cavity; and
at least one enhancing structure associated with said analyte-shaped cavity. - View Dependent Claims (7, 10)
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8. A biochip comprising:
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a substrate;
an array comprising a plurality of defined areas on said substrate, each of said defined areas having at least one analyte-shaped cavity thereon; and
at least one enhancing structure associated with said cavity. - View Dependent Claims (9)
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11. A system for analyte detection, comprising:
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a substrate having a plurality of defined areas thereon, each of said areas having;
at least one enhancing structure thereon; and
at least one analyte-shaped cavity;
wherein each of said plurality of analyte-shaped cavities associated with each of said defined areas has a preferential analyte binding affinity different from the binding affinities of analyte-shaped cavities associated with different defined areas;
an identifier for at least one of said defined areas; and
a detector associated with a defined area. - View Dependent Claims (12)
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13. A method for analyte detection, comprising:
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providing an enhancing structure;
providing an analyte-shaped cavity near said enhancing structure;
exposing said analyte to said analyte-shaped cavity to form an analyte analyte-shaped cavity complex; and
detecting a Raman spectral feature associated with said analyte receptor complex. - View Dependent Claims (14)
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15. A method for manufacturing a matrix for analyte detection, comprising the steps of:
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providing an analyte within a polymerizable matrix;
adding to said polymerizable matrix a catalyst and a plurality of enhancing structures;
permitting said polymerizable matrix to polymerize forming a polymer matrix;
removing said analytes from said polymer matrix. - View Dependent Claims (17)
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16. A method of manufacturing a matrix for analyte detection, comprising the steps of:
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providing a substrate having a surface and an analyte molecule attached thereto;
providing a polymerizable matrix having a plurality of enhancing structures therein;
overlaying said polymerizable matrix onto said substrate and said analyte molecule; and
permitting said polymerizable matrix to polymerize forming a polymer matrix having analyte-shaped cavities on one surface of said matrix. - View Dependent Claims (18)
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19. A Raman reader comprising:
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a light source;
a matrix array having enhancing structures and analyte-shaped cavities thereon;
a holder for positioning said matrix array in relation to said light source;
a light detector. - View Dependent Claims (20, 21, 22)
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23. A method for detecting an analyte on a biochip, comprising the steps of:
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providing a biochip having at least one defined area thereon having at least one analyte-shaped cavity thereon;
providing a Raman reader;
exposing said biochip to a solution containing an analyte;
permitting said analyte to be retained by said analyte-shaped cavity forming an analyte analyte-shaped cavity complex;
exposing said defined area to incident light; and
collecting light emitted from a defined area.
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Specification