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Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds for 8-color DNA sequencing

  • US 20030180769A1
  • Filed: 02/05/2003
  • Published: 09/25/2003
  • Est. Priority Date: 02/05/2002
  • Status: Abandoned Application
First Claim
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1. A method of sequencing a sense strand and an antisense strand of a double-stranded polynucleotide comprising:

  • i) denaturing the double-stranded polynucleotide to provide the sense strand and the antisense strand;

    ii) reacting the sense strand with a first set of four differentially labeled polynucleotides;

    iii) reacting the antisense strand with a second set of four differentially labeled polynucleotides;

    iv) identifying each of the eight polynucleotides by a fluorescence or an absorption spectrum of the fluorophore; and

    v) determining the sequence of the sense strand from the polynucleotides differentially labeled with the first set of fluorophores and the sequence of the antisense strand from the polynucleotides differentially labeled with the second set of fluorophores.

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