Direct immunosensor assay
First Claim
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1. A disposable device for use in detecting a target antigen in a fluid sample, the device comprising a reaction chamber;
- an immobilized antibody fixed within the reaction chamber;
a reporter complex comprising a probe and a reporter complex antigen, wherein the probe is linked to the reporter complex antigen, wherein the reporter complex antigen is bound to the immobilized antibody, and wherein the reporter complex antigen binds less strongly than the target antigen to the immobilized antibody;
a detection chamber;
a sample ingress to the reaction chamber; and
a sample passageway between the reaction chamber and the detection chamber.
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Abstract
This invention describes a quantitative, inexpensive, disposable immunosensor that requires no wash steps and thus generates no liquid waste. Moreover, in preferred embodiments of the sensor no timing steps are required of the user, and the sensor can be readily adapted to antigen-antibody interactions over a wide kinetic range.
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Citations
57 Claims
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1. A disposable device for use in detecting a target antigen in a fluid sample, the device comprising a reaction chamber;
- an immobilized antibody fixed within the reaction chamber;
a reporter complex comprising a probe and a reporter complex antigen, wherein the probe is linked to the reporter complex antigen, wherein the reporter complex antigen is bound to the immobilized antibody, and wherein the reporter complex antigen binds less strongly than the target antigen to the immobilized antibody;
a detection chamber;
a sample ingress to the reaction chamber; and
a sample passageway between the reaction chamber and the detection chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
- an immobilized antibody fixed within the reaction chamber;
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53. A method for determining an amount of a target antigen in a fluid sample, the method comprising the steps of:
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placing the fluid sample in a reaction chamber containing an immobilized antibody and a reporter complex comprising a probe linked to a reporter complex antigen, wherein the antibody is fixed within the reaction chamber, wherein the reporter complex antigen is bound to the immobilized antibody, and wherein the reporter complex antigen binds less strongly than the target antigen to the immobilized antibody;
dissociating a portion of the reporter complex antigen from the immobilized antibody into the fluid sample;
binding a portion of the target antigen to the immobilized antibody;
transferring the fluid sample to a detection chamber; and
determining an amount of reporter complex in the fluid sample, wherein the amount of reporter complex is indicative of the amount of target antigen initially in the fluid sample. - View Dependent Claims (54, 55, 56, 57)
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Specification