Cell growth surface on a polymeric substrate

• US 20030180903A1
• Filed: 03/05/2003
• Published: 09/25/2003
• Est. Priority Date: 09/04/2001
• Status: Active Grant

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Wang, Hongming, Gagnon, Paul E., Shen, Zhong-he, Bryhan, Marie D., Lachance, Oliva V.
• Granted Patent: US 7,579,179 B2
• Time in Patent Office: Days
• US Class Current: 435/180
• CPC Class Codes: B05D 3/144 of polymeric substrates
• CPC Class Codes: B05D 7/22 to internal surfaces, e.g. of tubes
• CPC Class Codes: B29C 59/14 by plasma treatment plasma tubes per se H01J
• CPC Class Codes: B29C 59/142 of profiled articles, e.g. hollow or tubular articles

Abstract

A product exhibiting an improved cell growth surface and cell attachment. According to the present invention, a stream of plasma is comprised of activated gaseous species generated by a microwave source. This stream is directed at the surface of a polymer substrate in a controlled fashion such that the surface is imparted with attributes for cell adhesion far superior to that of untreated polymer or polymer treated by other methods.

Claims

1. A polymer substrate having a working surface upon which cells can be cultured wherein the surface oxygen content is at least 25 percent as measured by electron microscopy for chemical analysis at depth of about 50 Angstroms.

2. The substrate of claim 1 wherein said polymer is polystyrene.

3. The substrate of claim 1, wherein the substrate includes at least a portion of a roller bottle, a flask, or a multiwell plate.

4. A polymer substrate having a working surface upon which cells can be cultured wherein the surface oxygen content is at least 20 percent at a depth of about 80 Angstroms from the surface as measured by electron microscopy for chemical analysis.

5. The substrate of claim 4, wherein the oxygen content is at least 15% at a depth of about 110 nm from the surface.

6. The substrate of claim 4, wherein the substrate includes at least a portion of a roller bottle, a flask, or a multiwell plate.

7. The substrate of claim 5, wherein the substrate includes at least a portion of a roller bottle, a flask, or a multiwell plate.