Biosensor and method for analyzing blood components using it
First Claim
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1. A biosensor made of a dried porous material, said biosensor comprising:
- a sample introductory part for introducing a sample solution; and
a developing layer for developing the sample solution, wherein the developing layer includes;
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution; and
a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, and an amount of marker reagent bound in the reagent immobilization part is measured, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein a retiform structure is arranged in the sample introductory part.
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Abstract
An object of the present invention is to provide a biosensor which enables a measurement with a small amount of specimen, for which whole blood with a reduced influence of blood cell can be used without previously separating blood plasma components from the blood, in an analysis of a component in the blood.
The biosensor comprises a sample introductory part (11) to which a sample solution is applied, a space forming material (9) for forming the sample introductory part, a part (10) where a cell contraction agent for causing cellular components in the sample solution to constrict is held, a sample holding part (6) where the sample solution is held, a developing layer (2) for developing the sample solution, a marker reagent holding part (3) where a marker reagent is held, and a reagent immobilization part (5) as an area on the developing layer (2) where a specific protein is held. Further, a retiform structure (7) is provided between the space forming material (9) and the developing layer (2).
12 Citations
37 Claims
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1. A biosensor made of a dried porous material,
said biosensor comprising: - a sample introductory part for introducing a sample solution; and
a developing layer for developing the sample solution, whereinthe developing layer includes;
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution; and
a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, andan amount of marker reagent bound in the reagent immobilization part is measured, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein a retiform structure is arranged in the sample introductory part. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- a sample introductory part for introducing a sample solution; and
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2. A biosensor made of a dried porous material,
said biosensor comprising: - a developing layer for developing a sample solution;
a space forming part for forming a space on the developing layer; and
a sample introductory part having a cavity in which the sample solution flows, in the formed space, whereinthe developing layer includes;
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution; and
a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, andan amount of marker reagent bound in the reagent immobilization part is measured, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein a retiform structure is arranged in the sample introductory part.
- a developing layer for developing a sample solution;
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3. A biosensor made of a dried porous material,
said biosensor comprising: - a developing layer for developing a sample solution;
a space forming part for forming a space on the developing layer; and
a sample introductory part having a cavity in which the sample solution flows, in the formed space, whereina reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the flow of the sample solution is provided in the cavity, and the developing layer includes a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution is developed on the developing layer while eluting the marker reagent, and reaches the reagent immobilization part, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, and an amount of marker reagent bound in the reagent immobilization part is measured, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, wherein a retiform structure is arranged in the sample introductory part.
- a developing layer for developing a sample solution;
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20. A method for analyzing a constituent of blood by employing a biosensor made of a dried porous material,
said biosensor comprising: - a sample introductory part for introducing a sample solution; and
a developing layer for developing the sample solution, whereinthe developing layer includes;
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution; and
a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, andsaid analysis method comprising;
measuring an amount of marker reagent bound in the reagent immobilization part, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, whereina retiform structure is arranged in the sample introductory part, and the blood is developed. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
- a sample introductory part for introducing a sample solution; and
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21. A method for analyzing a constituent of blood by employing a biosensor made of a dried porous material,
said biosensor comprising: - a developing layer for developing a sample solution;
a space forming part for forming a space on the developing layer; and
a sample introductory part having a cavity in which the sample solution flows, in the formed space, whereinthe developing layer includes;
a marker reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the development of the sample solution; and
a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution permeates the developing layer to reach the marker reagent holding part, and moves to the reagent immobilization part while eluting the marker reagent, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, andsaid analysis method comprising;
measuring an amount of marker reagent bound in the reagent immobilization part, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, whereina retiform structure is arranged in the sample introductory part, and the blood is developed.
- a developing layer for developing a sample solution;
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22. A method for analyzing a constituent of blood by employing a biosensor made of a dried porous material,
said biosensor comprising: - a developing layer for developing a sample solution;
a space forming part for forming a space on the developing layer; and
a sample introductory part having a cavity in which the sample solution flows, in the formed space, whereina reagent holding part where a marker reagent is held in a dry state so that it can be eluted by the flow of the sample solution is provided in the cavity, and the developing layer includes a reagent immobilization part where a reagent which can bind to an analyte and is involved in a reaction is immobilized so that it is not eluted, and when the sample solution is introduced to the sample introductory part, the sample solution is developed on the developing layer while eluting the marker reagent, and reaches the reagent immobilization part, whereby the reaction among the analyte, the marker reagent, and the immobilized reagent occurs, and said analysis method comprising;
measuring an amount of the marker reagent bound in the reagent immobilization part, thereby qualitatively or quantitatively measuring the analyte included in the sample solution, whereina retiform structure is arranged in the sample introductory part, and the blood is developed.
- a developing layer for developing a sample solution;
Specification
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Current AssigneePanasonic Corporation (Panasonic Holdings Corporation)
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Original AssigneePanasonic Corporation (Panasonic Holdings Corporation)
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InventorsKitawaki, Fumuhisa, Tanaka, Hirotaka, Takahashi, Mie, Nadaoka, Masataka
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Application NumberUS10/398,711Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/7.32CPC Class CodesG01N 33/54388 based on lateral flowG01N 33/558 using diffusion or migratio...
